The thermodynamics of lanthanide ion binding to adriamycin

R. E. Lenkinski, S. Sierke

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We have examined the thermodynamics of lanthanide ion binding to adriamycin by monitoring the effects of variations in temperature on the dissociation constants of various lanthanide ion complexes of the drug. These constants were obtained by analyzing the extent of quenching of the fluorecence of adriamycin in the presence of lanthanide ions in terms of an equilibrium binding process. Our binding model included the following features, all of which are supported by evidence derived from previous published reports, vide infra. The lanthanides form 1:1 complexes with adriamycin. The binding is dependent on the pH of the solution, indicating that only the nonprotonated amine form of the drug participates in lanthanide ion binding. And finally the drug self-associates in solution to for a dimeric species. Our present results indicate that the binding process is almost completely independent of temperature, indicating that the enthalpy of complex formation is extremely small. The entropy terms are consistent with the formation of a complex in which the adriamycin acts as a bidentate ligand. Our results suggest that the lanthanide complexes are isostructural, at least as far as the adriamycin is concerned, throughout the lanthanide series.

Original languageEnglish (US)
Pages (from-to)59-67
Number of pages9
JournalJournal of Inorganic Biochemistry
Volume24
Issue number1
DOIs
StatePublished - 1985

Fingerprint

Lanthanoid Series Elements
Thermodynamics
Doxorubicin
Ions
Pharmaceutical Preparations
Temperature
Entropy
Amines
Enthalpy
Quenching
Ligands
Monitoring

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

Cite this

The thermodynamics of lanthanide ion binding to adriamycin. / Lenkinski, R. E.; Sierke, S.

In: Journal of Inorganic Biochemistry, Vol. 24, No. 1, 1985, p. 59-67.

Research output: Contribution to journalArticle

Lenkinski, R. E. ; Sierke, S. / The thermodynamics of lanthanide ion binding to adriamycin. In: Journal of Inorganic Biochemistry. 1985 ; Vol. 24, No. 1. pp. 59-67.
@article{f4db67116e2b4a9096433919fc7e9b3c,
title = "The thermodynamics of lanthanide ion binding to adriamycin",
abstract = "We have examined the thermodynamics of lanthanide ion binding to adriamycin by monitoring the effects of variations in temperature on the dissociation constants of various lanthanide ion complexes of the drug. These constants were obtained by analyzing the extent of quenching of the fluorecence of adriamycin in the presence of lanthanide ions in terms of an equilibrium binding process. Our binding model included the following features, all of which are supported by evidence derived from previous published reports, vide infra. The lanthanides form 1:1 complexes with adriamycin. The binding is dependent on the pH of the solution, indicating that only the nonprotonated amine form of the drug participates in lanthanide ion binding. And finally the drug self-associates in solution to for a dimeric species. Our present results indicate that the binding process is almost completely independent of temperature, indicating that the enthalpy of complex formation is extremely small. The entropy terms are consistent with the formation of a complex in which the adriamycin acts as a bidentate ligand. Our results suggest that the lanthanide complexes are isostructural, at least as far as the adriamycin is concerned, throughout the lanthanide series.",
author = "Lenkinski, {R. E.} and S. Sierke",
year = "1985",
doi = "10.1016/0162-0134(85)85014-5",
language = "English (US)",
volume = "24",
pages = "59--67",
journal = "Journal of Inorganic Biochemistry",
issn = "0162-0134",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - The thermodynamics of lanthanide ion binding to adriamycin

AU - Lenkinski, R. E.

AU - Sierke, S.

PY - 1985

Y1 - 1985

N2 - We have examined the thermodynamics of lanthanide ion binding to adriamycin by monitoring the effects of variations in temperature on the dissociation constants of various lanthanide ion complexes of the drug. These constants were obtained by analyzing the extent of quenching of the fluorecence of adriamycin in the presence of lanthanide ions in terms of an equilibrium binding process. Our binding model included the following features, all of which are supported by evidence derived from previous published reports, vide infra. The lanthanides form 1:1 complexes with adriamycin. The binding is dependent on the pH of the solution, indicating that only the nonprotonated amine form of the drug participates in lanthanide ion binding. And finally the drug self-associates in solution to for a dimeric species. Our present results indicate that the binding process is almost completely independent of temperature, indicating that the enthalpy of complex formation is extremely small. The entropy terms are consistent with the formation of a complex in which the adriamycin acts as a bidentate ligand. Our results suggest that the lanthanide complexes are isostructural, at least as far as the adriamycin is concerned, throughout the lanthanide series.

AB - We have examined the thermodynamics of lanthanide ion binding to adriamycin by monitoring the effects of variations in temperature on the dissociation constants of various lanthanide ion complexes of the drug. These constants were obtained by analyzing the extent of quenching of the fluorecence of adriamycin in the presence of lanthanide ions in terms of an equilibrium binding process. Our binding model included the following features, all of which are supported by evidence derived from previous published reports, vide infra. The lanthanides form 1:1 complexes with adriamycin. The binding is dependent on the pH of the solution, indicating that only the nonprotonated amine form of the drug participates in lanthanide ion binding. And finally the drug self-associates in solution to for a dimeric species. Our present results indicate that the binding process is almost completely independent of temperature, indicating that the enthalpy of complex formation is extremely small. The entropy terms are consistent with the formation of a complex in which the adriamycin acts as a bidentate ligand. Our results suggest that the lanthanide complexes are isostructural, at least as far as the adriamycin is concerned, throughout the lanthanide series.

UR - http://www.scopus.com/inward/record.url?scp=0022187682&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022187682&partnerID=8YFLogxK

U2 - 10.1016/0162-0134(85)85014-5

DO - 10.1016/0162-0134(85)85014-5

M3 - Article

AN - SCOPUS:0022187682

VL - 24

SP - 59

EP - 67

JO - Journal of Inorganic Biochemistry

JF - Journal of Inorganic Biochemistry

SN - 0162-0134

IS - 1

ER -