The three extra-cellular zinc metalloproteinases of Streptococcus pneumoniae have a different impact on virulence in mice

Damiana Chiavolini, Guido Memmi, Tiziana Maggi, Francesco Iannelli, Gianni Pozzi, Marco R. Oggioni

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Background: Streptococcus pneumoniae possesses large zinc metalloproteinases on its surface. To analyse the importance in virulence of three of these metalloproteinases, intranasal challenge of MF1 outbred mice was carried out using a range of infecting doses of wild type and knock-out pneumococcal mutant strains, in order to compare mice survival. Results: Observation of survival percentages over time and detection of LD50s of knock out)mutants in the proteinase genes in comparison to the type 4 TIGR4 wild type strain revealed two major aspects: i) Iga and ZmpB, present in all strains of S. pneumoniae, strongly contribute to virulence in mice; (ii) ZmpC, only present in about 25% of pneumococcal strains, has a lower influence on virulence in mice. Conclusions: These data suggest Iga, ZmpB and ZmpC as candidate surface proteins responsible for pneumococcal infection and potentially involved in distinct stages of pneumococcal disease.

Original languageEnglish (US)
Article number1
Pages (from-to)1-9
Number of pages9
JournalBMC Microbiology
Volume3
DOIs
StatePublished - Jul 3 2003

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Metalloproteases
Streptococcus pneumoniae
Virulence
Zinc
Pneumococcal Infections
Membrane Proteins
Peptide Hydrolases
Observation
Genes

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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The three extra-cellular zinc metalloproteinases of Streptococcus pneumoniae have a different impact on virulence in mice. / Chiavolini, Damiana; Memmi, Guido; Maggi, Tiziana; Iannelli, Francesco; Pozzi, Gianni; Oggioni, Marco R.

In: BMC Microbiology, Vol. 3, 1, 03.07.2003, p. 1-9.

Research output: Contribution to journalArticle

Chiavolini, Damiana ; Memmi, Guido ; Maggi, Tiziana ; Iannelli, Francesco ; Pozzi, Gianni ; Oggioni, Marco R. / The three extra-cellular zinc metalloproteinases of Streptococcus pneumoniae have a different impact on virulence in mice. In: BMC Microbiology. 2003 ; Vol. 3. pp. 1-9.
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