TY - JOUR
T1 - The toxicity of chemically deglycosylated ricin A-chain in mice
AU - Soler-Rodriguez, A. M.
AU - Uhr, J. W.
AU - Richardson, J.
AU - Vitetta, E. S.
N1 - Funding Information:
Acknowledgements -- We thank Ms S. Shanahan, Mr F. Catalina and Ms B. K. Morrison for their excellent technical assistance. The authors greatly appreciate the blood chemistry data provided by Dr R. S, Putman, and the serum TNF-a determinations by Dr X, SJ.ez-Llorens. Finally, we thank Ms R. Reiber and Ms C. Baselski for expert secretarial assistance. Supported by NIH grants CA28149, CA41081 and RR00890.
PY - 1992/2
Y1 - 1992/2
N2 - Tumor-reactive antibodies coupled to ricin or its A-chain (immunotoxins) have been used in rodents and humans to treat a variety of neoplastic diseases. Side-effects of such treatment include hepatotoxicity, vascular leak syndrome, myalgia and low grade fever. At high doses, severe toxicities include liver damage, pulmonary edema, aphasia, rhabdomyolysis and kidney failure. There have been a limited number of toxicologic studies on uncoupled ricin or its A-chain and none on deglycosylated A-chain. Since the latter has been utilized in "second generation" immunotoxins, the current studies were carried out to evaluate the toxicities induced by deglycosylated ricin A-chain (dgA) in mice. The administration of dgA to normal BALB/c mice causes early (24 h) weight loss and late (10 day) accumumulation of ascites. These effects could be partially altered by changing the route of injection of dgA from i.v. to i.p. Thus, i.p. administration caused weight loss but not ascites, whereas i.v. administration caused both. Weight loss was associated with reduced fluid intake by the treated mice, and was not associated with increased levels of serum TNF-α. SCID mice injected with the same dose of dgA as normal BALB/c mice developed ascites, but it was of lesser severity, suggesting that a functional immune system, differences in microbial flora, or strain differences may be involved in the development of ascites.
AB - Tumor-reactive antibodies coupled to ricin or its A-chain (immunotoxins) have been used in rodents and humans to treat a variety of neoplastic diseases. Side-effects of such treatment include hepatotoxicity, vascular leak syndrome, myalgia and low grade fever. At high doses, severe toxicities include liver damage, pulmonary edema, aphasia, rhabdomyolysis and kidney failure. There have been a limited number of toxicologic studies on uncoupled ricin or its A-chain and none on deglycosylated A-chain. Since the latter has been utilized in "second generation" immunotoxins, the current studies were carried out to evaluate the toxicities induced by deglycosylated ricin A-chain (dgA) in mice. The administration of dgA to normal BALB/c mice causes early (24 h) weight loss and late (10 day) accumumulation of ascites. These effects could be partially altered by changing the route of injection of dgA from i.v. to i.p. Thus, i.p. administration caused weight loss but not ascites, whereas i.v. administration caused both. Weight loss was associated with reduced fluid intake by the treated mice, and was not associated with increased levels of serum TNF-α. SCID mice injected with the same dose of dgA as normal BALB/c mice developed ascites, but it was of lesser severity, suggesting that a functional immune system, differences in microbial flora, or strain differences may be involved in the development of ascites.
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U2 - 10.1016/0192-0561(92)90041-I
DO - 10.1016/0192-0561(92)90041-I
M3 - Article
C2 - 1624227
AN - SCOPUS:0026503802
SN - 0192-0561
VL - 14
SP - 281
EP - 291
JO - International Journal of Immunopharmacology
JF - International Journal of Immunopharmacology
IS - 2
ER -