The Tpl2 mutation Sluggish impairs type I IFN production and increases susceptibility to group B streptococcal disease

Nengming Xiao, Celine Eidenschenk, Philippe Krebs, Katharina Brandl, Amanda L. Blasius, Yu Xia, Kevin Khovananth, Nora G. Smart, Bruce Beutler

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Sluggish was identified in a population of third generation mice descended from N-ethyl-N-nitrosourea-mutagenized sires. Macrophages from homozygotes exhibited impaired TNF-α production in response to all TLR ligands tested and displayed impaired type I IFN production in response to TLR7 and TLR9 stimulations. The phenotype was confined to a critical region on mouse chromosome 18 and then ascribed to a T to A transversion in the acceptor splice site of intron 4 at position 13346 of the Map3k8 gene, resulting in defective splicing. The Map3k8Sluggish mutation does not result in susceptibility to viral infections, but Sluggish mice displayed high susceptibility to group B streptococcus infection, with impaired TNF-α and type I IFN production in infected macrophages. Our data demonstrate that the encoded protein kinase Tpl2 plays an essential role in cell signaling in the immune response to certain pathogens.

Original languageEnglish (US)
Pages (from-to)7975-7983
Number of pages9
JournalJournal of Immunology
Volume183
Issue number12
DOIs
StatePublished - Dec 15 2009

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Xiao, N., Eidenschenk, C., Krebs, P., Brandl, K., Blasius, A. L., Xia, Y., Khovananth, K., Smart, N. G., & Beutler, B. (2009). The Tpl2 mutation Sluggish impairs type I IFN production and increases susceptibility to group B streptococcal disease. Journal of Immunology, 183(12), 7975-7983. https://doi.org/10.4049/jimmunol.0902718