The translin-TRAX complex (C3PO) is a ribonuclease in tRNA processing

Liande Li, Weifeng Gu, Chunyang Liang, Qinghua Liu, Craig C. Mello, Yi Liu

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Conserved translin-TRAX complexes, also known as C3POs, have been implicated in many biological processes, but how they function remains unclear. Recently, C3PO was shown to be an endoRNase that promotes RNA interference (RNAi) in animal cells. Here, we show that C3PO does not play a significant role in RNAi in the filamentous fungus Neurospora crassa. Instead, the Neurospora C3PO functions as an RNase that removes the 5'2 pre-tRNA fragments after the processing of pre-tRNAs by RNase P. In addition, translin and trax mutants have elevated levels of tRNA and protein translation and are more resistant to a cell death-inducing agent. Finally, we show that C3PO is also involved in tRNA processing in mouse embryonic fibroblast cells. This study identifies the endogenous RNA substrates of C3PO and provides a potential explanation for its roles in apparently diverse biological processes.

Original languageEnglish (US)
Pages (from-to)824-830
Number of pages7
JournalNature Structural and Molecular Biology
Volume19
Issue number8
DOIs
StatePublished - Aug 2012

Fingerprint

Biological Phenomena
RNA Precursors
Ribonucleases
RNA Interference
Transfer RNA
Ribonuclease P
Neurospora
Neurospora crassa
Protein Biosynthesis
Fungi
Cell Death
Fibroblasts
RNA
angiogenin

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

The translin-TRAX complex (C3PO) is a ribonuclease in tRNA processing. / Li, Liande; Gu, Weifeng; Liang, Chunyang; Liu, Qinghua; Mello, Craig C.; Liu, Yi.

In: Nature Structural and Molecular Biology, Vol. 19, No. 8, 08.2012, p. 824-830.

Research output: Contribution to journalArticle

Li, Liande ; Gu, Weifeng ; Liang, Chunyang ; Liu, Qinghua ; Mello, Craig C. ; Liu, Yi. / The translin-TRAX complex (C3PO) is a ribonuclease in tRNA processing. In: Nature Structural and Molecular Biology. 2012 ; Vol. 19, No. 8. pp. 824-830.
@article{1a3f33f7e7df4cae852f21ababbb9b58,
title = "The translin-TRAX complex (C3PO) is a ribonuclease in tRNA processing",
abstract = "Conserved translin-TRAX complexes, also known as C3POs, have been implicated in many biological processes, but how they function remains unclear. Recently, C3PO was shown to be an endoRNase that promotes RNA interference (RNAi) in animal cells. Here, we show that C3PO does not play a significant role in RNAi in the filamentous fungus Neurospora crassa. Instead, the Neurospora C3PO functions as an RNase that removes the 5'2 pre-tRNA fragments after the processing of pre-tRNAs by RNase P. In addition, translin and trax mutants have elevated levels of tRNA and protein translation and are more resistant to a cell death-inducing agent. Finally, we show that C3PO is also involved in tRNA processing in mouse embryonic fibroblast cells. This study identifies the endogenous RNA substrates of C3PO and provides a potential explanation for its roles in apparently diverse biological processes.",
author = "Liande Li and Weifeng Gu and Chunyang Liang and Qinghua Liu and Mello, {Craig C.} and Yi Liu",
year = "2012",
month = "8",
doi = "10.1038/nsmb.2337",
language = "English (US)",
volume = "19",
pages = "824--830",
journal = "Nature Structural and Molecular Biology",
issn = "1545-9993",
publisher = "Nature Publishing Group",
number = "8",

}

TY - JOUR

T1 - The translin-TRAX complex (C3PO) is a ribonuclease in tRNA processing

AU - Li, Liande

AU - Gu, Weifeng

AU - Liang, Chunyang

AU - Liu, Qinghua

AU - Mello, Craig C.

AU - Liu, Yi

PY - 2012/8

Y1 - 2012/8

N2 - Conserved translin-TRAX complexes, also known as C3POs, have been implicated in many biological processes, but how they function remains unclear. Recently, C3PO was shown to be an endoRNase that promotes RNA interference (RNAi) in animal cells. Here, we show that C3PO does not play a significant role in RNAi in the filamentous fungus Neurospora crassa. Instead, the Neurospora C3PO functions as an RNase that removes the 5'2 pre-tRNA fragments after the processing of pre-tRNAs by RNase P. In addition, translin and trax mutants have elevated levels of tRNA and protein translation and are more resistant to a cell death-inducing agent. Finally, we show that C3PO is also involved in tRNA processing in mouse embryonic fibroblast cells. This study identifies the endogenous RNA substrates of C3PO and provides a potential explanation for its roles in apparently diverse biological processes.

AB - Conserved translin-TRAX complexes, also known as C3POs, have been implicated in many biological processes, but how they function remains unclear. Recently, C3PO was shown to be an endoRNase that promotes RNA interference (RNAi) in animal cells. Here, we show that C3PO does not play a significant role in RNAi in the filamentous fungus Neurospora crassa. Instead, the Neurospora C3PO functions as an RNase that removes the 5'2 pre-tRNA fragments after the processing of pre-tRNAs by RNase P. In addition, translin and trax mutants have elevated levels of tRNA and protein translation and are more resistant to a cell death-inducing agent. Finally, we show that C3PO is also involved in tRNA processing in mouse embryonic fibroblast cells. This study identifies the endogenous RNA substrates of C3PO and provides a potential explanation for its roles in apparently diverse biological processes.

UR - http://www.scopus.com/inward/record.url?scp=84864690535&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84864690535&partnerID=8YFLogxK

U2 - 10.1038/nsmb.2337

DO - 10.1038/nsmb.2337

M3 - Article

VL - 19

SP - 824

EP - 830

JO - Nature Structural and Molecular Biology

JF - Nature Structural and Molecular Biology

SN - 1545-9993

IS - 8

ER -