The trk proto-oncogene product: A signal transducing receptor for nerve growth factor

David R. Kaplan, Barbara L. Hempstead, Dionisio Martin-Zanca, Moses V. Chao, Luis F. Parada

Research output: Contribution to journalArticle

1067 Citations (Scopus)

Abstract

The trk proto-oncogene encodes a 140-kilodalton, membrane-spanning protein tyrosine kinase (p140prototrk) that is expressed only in neural tissues. Nerve growth factor (NGF) stimulates phosphorylation of p140prototrk in neural cell lines and in embryonic dorsal root ganglia. Affinity cross-linking and equilibrium binding experiments with 125I-labeled NGF indicate that p140prototrk binds NGF specifically in cultured cells with a dissociation constant of 10-9 molar. The identification of p140prototrk as an NGF receptor indicates that this protein participates in the primary signal transduction mechanism of NGF.

Original languageEnglish (US)
Pages (from-to)554-558
Number of pages5
JournalScience
Volume252
Issue number5005
StatePublished - Apr 26 1991

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Nerve Growth Factor Receptor
Proto-Oncogenes
Oncogene Proteins
Nerve Growth Factor
Spinal Ganglia
Protein-Tyrosine Kinases
Cultured Cells
Signal Transduction
Membrane Proteins
Phosphorylation
Cell Line
Proteins

ASJC Scopus subject areas

  • General

Cite this

Kaplan, D. R., Hempstead, B. L., Martin-Zanca, D., Chao, M. V., & Parada, L. F. (1991). The trk proto-oncogene product: A signal transducing receptor for nerve growth factor. Science, 252(5005), 554-558.

The trk proto-oncogene product : A signal transducing receptor for nerve growth factor. / Kaplan, David R.; Hempstead, Barbara L.; Martin-Zanca, Dionisio; Chao, Moses V.; Parada, Luis F.

In: Science, Vol. 252, No. 5005, 26.04.1991, p. 554-558.

Research output: Contribution to journalArticle

Kaplan, DR, Hempstead, BL, Martin-Zanca, D, Chao, MV & Parada, LF 1991, 'The trk proto-oncogene product: A signal transducing receptor for nerve growth factor', Science, vol. 252, no. 5005, pp. 554-558.
Kaplan DR, Hempstead BL, Martin-Zanca D, Chao MV, Parada LF. The trk proto-oncogene product: A signal transducing receptor for nerve growth factor. Science. 1991 Apr 26;252(5005):554-558.
Kaplan, David R. ; Hempstead, Barbara L. ; Martin-Zanca, Dionisio ; Chao, Moses V. ; Parada, Luis F. / The trk proto-oncogene product : A signal transducing receptor for nerve growth factor. In: Science. 1991 ; Vol. 252, No. 5005. pp. 554-558.
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