The two-component system QseEF and the membrane protein QseG link adrenergic and stress sensing to bacterial pathogenesis

Nicola C. Reading, David A. Rasko, Alfredo G. Torres, Vanessa Sperandio

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Bacterial pathogens sense host cues to activate expression of virulence genes. Most ofthese signals are sensed through histidine kinases (HKs), which comprise the main sensory mechanism in bacteria. The host stress hormones epinephrine (Epi) and norepinephrine are sensed through the QseC HK, which initiates a complex signaling cascadetoregulate virulence gene expression in enterohemorrhagic Escherichia coli (EHEC). Epi signaling through QseC activates expression of the genes encoding the QseEF 2-component system. QseE is an HK, and QseF is a response regulator. Here,weshow that QseEisa second bacterial adrenergic receptor that gauges the stress signals Epi, sulfate, and phosphate. The qseEF genes are organized within an unusual operonic structure, in that a gene is encoded between qseE and qseF. This gene was renamed qseG, and it was shown to encode an outer membrane (OM) protein. EHEC uses a type III secretion system (TTSS) to translocate effector proteins to the epithelial cells that rearrange the host cytoskeleton to form pedestal-like structures that cup the bacterium. QseE, QseG, and QseF are necessary for pedestal formation. Although QseE and QseF are involvedin the transcriptional control of genes necessary for pedestal formation, QseG is necessary for translocation of effectors into epithelial cells. QseG is an OM protein necessary for translocation of TTSS effectors that also works in conjunction with a 2-component signaling system that senses host stress signals.

Original languageEnglish (US)
Pages (from-to)5889-5894
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number14
DOIs
StatePublished - Apr 7 2009

Fingerprint

Adrenergic Agents
Membrane Proteins
Enterohemorrhagic Escherichia coli
Gene Expression
Epinephrine
Genes
Virulence
Epithelial Cells
Bacteria
Protein Transport
Cytoskeleton
Adrenergic Receptors
Cues
Norepinephrine
Phosphates
Hormones
Histidine Kinase
Proteins

Keywords

  • Enterohemorrhagic E. coli
  • Epinephrine
  • Interkingdom signaling type III secretion

ASJC Scopus subject areas

  • General

Cite this

The two-component system QseEF and the membrane protein QseG link adrenergic and stress sensing to bacterial pathogenesis. / Reading, Nicola C.; Rasko, David A.; Torres, Alfredo G.; Sperandio, Vanessa.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, No. 14, 07.04.2009, p. 5889-5894.

Research output: Contribution to journalArticle

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