The ultraviolet endonuclease of bacteriophage T4. Further characterization.

K. Minton, M. Durphy, R. Taylor, E. C. Friedberg

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The T4 ultraviolet endonuclease was previously shown to produce strand incisions (nicks) in ultraviolet-irradiated DNA on the 5' side of thymine dimers. The present studies demonstrate that the purified endonuclease creates 3'-OH and 5'-P termini at the sites of nicking. Photoreactivation of ultraviolet-sensitive sites, thereby demonstrating directly endonucleause has a molecular weight of approximately 18,000 and attacks ultraviolet-irradiated single-stranded Escherichia coli and M-13 DNA.

Original languageEnglish (US)
Pages (from-to)2823-2829
Number of pages7
JournalJournal of Biological Chemistry
Volume250
Issue number8
StatePublished - Apr 25 1975

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Bacteriophage T4
Bacteriophages
Endonucleases
Pyrimidine Dimers
DNA
Escherichia coli
Molecular Weight
Molecular weight

ASJC Scopus subject areas

  • Biochemistry

Cite this

The ultraviolet endonuclease of bacteriophage T4. Further characterization. / Minton, K.; Durphy, M.; Taylor, R.; Friedberg, E. C.

In: Journal of Biological Chemistry, Vol. 250, No. 8, 25.04.1975, p. 2823-2829.

Research output: Contribution to journalArticle

Minton, K. ; Durphy, M. ; Taylor, R. ; Friedberg, E. C. / The ultraviolet endonuclease of bacteriophage T4. Further characterization. In: Journal of Biological Chemistry. 1975 ; Vol. 250, No. 8. pp. 2823-2829.
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