The ultraviolet endonuclease of bacteriophage T4. Further characterization.

K. Minton, M. Durphy, R. Taylor, E. C. Friedberg

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

The T4 ultraviolet endonuclease was previously shown to produce strand incisions (nicks) in ultraviolet-irradiated DNA on the 5' side of thymine dimers. The present studies demonstrate that the purified endonuclease creates 3'-OH and 5'-P termini at the sites of nicking. Photoreactivation of ultraviolet-sensitive sites, thereby demonstrating directly endonucleause has a molecular weight of approximately 18,000 and attacks ultraviolet-irradiated single-stranded Escherichia coli and M-13 DNA.

Original languageEnglish (US)
Pages (from-to)2823-2829
Number of pages7
JournalJournal of Biological Chemistry
Volume250
Issue number8
StatePublished - Apr 25 1975

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'The ultraviolet endonuclease of bacteriophage T4. Further characterization.'. Together they form a unique fingerprint.

  • Cite this