The ultraviolet endonuclease of bacteriophage T4. Further characterization.

K. Minton; M. Durphy; R. Taylor; E. C. Friedberg

The ultraviolet endonuclease of bacteriophage T4. Further characterization.

K. Minton, M. Durphy, R. Taylor, E. C. Friedberg

Research output: Contribution to journal › Article › peer-review

Abstract

The T4 ultraviolet endonuclease was previously shown to produce strand incisions (nicks) in ultraviolet-irradiated DNA on the 5' side of thymine dimers. The present studies demonstrate that the purified endonuclease creates 3'-OH and 5'-P termini at the sites of nicking. Photoreactivation of ultraviolet-sensitive sites, thereby demonstrating directly endonucleause has a molecular weight of approximately 18,000 and attacks ultraviolet-irradiated single-stranded Escherichia coli and M-13 DNA.

Original language	English (US)
Pages (from-to)	2823-2829
Number of pages	7
Journal	Journal of Biological Chemistry
Volume	250
Issue number	8
State	Published - Apr 25 1975

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

Cite this

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abstract = "The T4 ultraviolet endonuclease was previously shown to produce strand incisions (nicks) in ultraviolet-irradiated DNA on the 5' side of thymine dimers. The present studies demonstrate that the purified endonuclease creates 3'-OH and 5'-P termini at the sites of nicking. Photoreactivation of ultraviolet-sensitive sites, thereby demonstrating directly endonucleause has a molecular weight of approximately 18,000 and attacks ultraviolet-irradiated single-stranded Escherichia coli and M-13 DNA.",

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AU - Durphy, M.

AU - Taylor, R.

AU - Friedberg, E. C.

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PY - 1975/4/25

Y1 - 1975/4/25

N2 - The T4 ultraviolet endonuclease was previously shown to produce strand incisions (nicks) in ultraviolet-irradiated DNA on the 5' side of thymine dimers. The present studies demonstrate that the purified endonuclease creates 3'-OH and 5'-P termini at the sites of nicking. Photoreactivation of ultraviolet-sensitive sites, thereby demonstrating directly endonucleause has a molecular weight of approximately 18,000 and attacks ultraviolet-irradiated single-stranded Escherichia coli and M-13 DNA.

AB - The T4 ultraviolet endonuclease was previously shown to produce strand incisions (nicks) in ultraviolet-irradiated DNA on the 5' side of thymine dimers. The present studies demonstrate that the purified endonuclease creates 3'-OH and 5'-P termini at the sites of nicking. Photoreactivation of ultraviolet-sensitive sites, thereby demonstrating directly endonucleause has a molecular weight of approximately 18,000 and attacks ultraviolet-irradiated single-stranded Escherichia coli and M-13 DNA.

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JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 8

ER -

The ultraviolet endonuclease of bacteriophage T4. Further characterization.

Abstract

ASJC Scopus subject areas

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