The unliganded long isoform of estrogen receptor beta stimulates brain ryanodine receptor single channel activity alongside with cytosolic Ca 2+

Volodymyr Rybalchenko, Michael A. Grillo, Matthew J. Gastinger, Nataliya Rybalchenko, Andrew J. Payne, Peter Koulen

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Ca2+ release from intracellular stores mediated by endoplasmic reticulum membrane ryanodine receptors (RyR) plays a key role in activating and synchronizing downstream Ca2+-dependent mechanisms, in different cells varying from apoptosis to nuclear transcription and development of defensive responses. Recently discovered, atypical "nongenomic" effects mediated by estrogen receptors (ER) include rapid Ca2+ release upon estrogen exposure in conditions implicitly suggesting involvement of RyRs. In the present study, we report various levels of colocalization between RyR type 2 (RyR2) and ER type β (ERβ) in the neuronal cell line HT-22, indicating a possible functional interaction. Electrophysiological analyses revealed a significant increase in single-channel ionic currents generated by mouse brain RyRs after application of the soluble monomer of the long form ERβ (ERβ 1). The effect was due to a strong increase in open probability of RyR higher open channel sublevels at cytosolic [Ca2+] concentrations of 100nM, suggesting a synergistic action of ERβ1 and Ca2+ in RyR activation, and a potential contribution to Ca 2+-induced Ca2+ release rather than to basal intracellular Ca2 concentration level at rest. This RyR/ERβ interaction has potential effects on cellular physiology, including roles of shorter ERβ isoforms and modulation of the RyR/ERβ complexes by exogenous estrogens.

Original languageEnglish (US)
Pages (from-to)326-341
Number of pages16
JournalJournal of Receptors and Signal Transduction
Volume29
Issue number6
DOIs
StatePublished - Dec 2009

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Estrogen Receptor beta
Ryanodine Receptor Calcium Release Channel
Estrogen Receptors
Brain
Protein Isoforms
Estrogens
Estrogen Receptor alpha
Physiology
Transcription
Ion Channels
Endoplasmic Reticulum
Monomers
Chemical activation
Cells
Modulation
Apoptosis
Membranes
Cell Line

Keywords

  • -estradiol
  • 17β
  • Electrophysiology
  • Endocrinology
  • Intracellular calcium stores
  • Nervous system
  • Neuron

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

The unliganded long isoform of estrogen receptor beta stimulates brain ryanodine receptor single channel activity alongside with cytosolic Ca 2+ . / Rybalchenko, Volodymyr; Grillo, Michael A.; Gastinger, Matthew J.; Rybalchenko, Nataliya; Payne, Andrew J.; Koulen, Peter.

In: Journal of Receptors and Signal Transduction, Vol. 29, No. 6, 12.2009, p. 326-341.

Research output: Contribution to journalArticle

Rybalchenko, Volodymyr ; Grillo, Michael A. ; Gastinger, Matthew J. ; Rybalchenko, Nataliya ; Payne, Andrew J. ; Koulen, Peter. / The unliganded long isoform of estrogen receptor beta stimulates brain ryanodine receptor single channel activity alongside with cytosolic Ca 2+ In: Journal of Receptors and Signal Transduction. 2009 ; Vol. 29, No. 6. pp. 326-341.
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