The unusual action of (R,S)-2-hydroxy-2-trifluoromethyl-trans-n-octadec-4-enoic acid on 5-lipoxygenase from potato tubers

I. A. Butovich, V. A. Soloshonok, V. P. Kukhar

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We found that (R,S)-2-hydroxy-2-trifluoromethyl-trans-n-octadec-4-enoic acid (HTFOA) is a powerful activator of 5-lipoxygenase from potato tubers. The degree of activation of the enzyme is proportional to the HTFOA concentration and is a maximum at about 0.1 mM independently of initial substrate concentration (25 μM or 0.1 mM). At greater concentrations of HTFOA, enzyme inhibition takes place. Enzyme activation is inversely proportional to the substrate (linoleic acid) concentration. The results may be explained by assuming that a regulatory center exists in the enzyme molecule, which shows affinity to both substances: activator and linoleic acid.

Original languageEnglish (US)
Pages (from-to)153-155
Number of pages3
JournalEuropean Journal of Biochemistry
Volume199
Issue number1
StatePublished - 1991

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Arachidonate 5-Lipoxygenase
Enzyme Activation
Linoleic Acid
Solanum tuberosum
Acids
Enzymes
Chemical activation
Enzyme inhibition
Substrates
Molecules
2-hydroxy-2-trifluoromethyloctadec-4-enoic acid

ASJC Scopus subject areas

  • Biochemistry

Cite this

The unusual action of (R,S)-2-hydroxy-2-trifluoromethyl-trans-n-octadec-4-enoic acid on 5-lipoxygenase from potato tubers. / Butovich, I. A.; Soloshonok, V. A.; Kukhar, V. P.

In: European Journal of Biochemistry, Vol. 199, No. 1, 1991, p. 153-155.

Research output: Contribution to journalArticle

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