The use of glucose oxidase as a generator of H2O2 in the enzymatic radioiodination of components of cell surfaces

Isaac Schenkein, Milton Levy, Jonathan W. Uhr

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Glucose oxidase generates H2O2 via the conversion of glucose to gluconolactone in the presence of molecular oxygen. Coupling of this reaction to the lactoperoxidase-catalyzed substitution of iodine for hydrogen on the phenolic side chains of proteins present on the surfaces of murine myeloma cells leads to a very marked increase in incorporation of radioactivity as well as a significant improvement of cell survival.

Original languageEnglish (US)
Pages (from-to)490-493
Number of pages4
JournalCellular Immunology
Volume5
Issue number3
DOIs
StatePublished - Nov 1972

ASJC Scopus subject areas

  • Immunology

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