The use of glucose oxidase as a generator of H2O2 in the enzymatic radioiodination of components of cell surfaces

Isaac Schenkein, Milton Levy, Jonathan W. Uhr

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

Glucose oxidase generates H2O2 via the conversion of glucose to gluconolactone in the presence of molecular oxygen. Coupling of this reaction to the lactoperoxidase-catalyzed substitution of iodine for hydrogen on the phenolic side chains of proteins present on the surfaces of murine myeloma cells leads to a very marked increase in incorporation of radioactivity as well as a significant improvement of cell survival.

Original languageEnglish (US)
Pages (from-to)490-493
Number of pages4
JournalCellular Immunology
Volume5
Issue number3
DOIs
Publication statusPublished - 1972

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ASJC Scopus subject areas

  • Cell Biology
  • Immunology

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