The use of glucose oxidase as a generator of H2O2 in the enzymatic radioiodination of components of cell surfaces

Isaac Schenkein; Milton Levy; Jonathan W. Uhr

doi:10.1016/0008-8749(72)90076-7

The use of glucose oxidase as a generator of H₂O₂ in the enzymatic radioiodination of components of cell surfaces

Isaac Schenkein, Milton Levy, Jonathan W. Uhr

Immunology

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

Glucose oxidase generates H₂O₂ via the conversion of glucose to gluconolactone in the presence of molecular oxygen. Coupling of this reaction to the lactoperoxidase-catalyzed substitution of iodine for hydrogen on the phenolic side chains of proteins present on the surfaces of murine myeloma cells leads to a very marked increase in incorporation of radioactivity as well as a significant improvement of cell survival.

Original language	English (US)
Pages (from-to)	490-493
Number of pages	4
Journal	Cellular Immunology
Volume	5
Issue number	3
DOIs	https://doi.org/10.1016/0008-8749(72)90076-7
State	Published - Nov 1972

ASJC Scopus subject areas

Immunology

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10.1016/0008-8749(72)90076-7

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title = "The use of glucose oxidase as a generator of H2O2 in the enzymatic radioiodination of components of cell surfaces",

abstract = "Glucose oxidase generates H2O2 via the conversion of glucose to gluconolactone in the presence of molecular oxygen. Coupling of this reaction to the lactoperoxidase-catalyzed substitution of iodine for hydrogen on the phenolic side chains of proteins present on the surfaces of murine myeloma cells leads to a very marked increase in incorporation of radioactivity as well as a significant improvement of cell survival.",

author = "Isaac Schenkein and Milton Levy and Uhr, {Jonathan W.}",

note = "Funding Information: work was done under the sponsorship of the Commission on Immunization Forces Epidemiological Board and was supported in part by the U.S. Army Development Command, Department of the Army, under Research Contract 9177 and in part by U.S. Public Health Service Grant Al-0834.",

year = "1972",

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T1 - The use of glucose oxidase as a generator of H2O2 in the enzymatic radioiodination of components of cell surfaces

AU - Schenkein, Isaac

AU - Levy, Milton

AU - Uhr, Jonathan W.

N1 - Funding Information: work was done under the sponsorship of the Commission on Immunization Forces Epidemiological Board and was supported in part by the U.S. Army Development Command, Department of the Army, under Research Contract 9177 and in part by U.S. Public Health Service Grant Al-0834.

PY - 1972/11

Y1 - 1972/11

N2 - Glucose oxidase generates H2O2 via the conversion of glucose to gluconolactone in the presence of molecular oxygen. Coupling of this reaction to the lactoperoxidase-catalyzed substitution of iodine for hydrogen on the phenolic side chains of proteins present on the surfaces of murine myeloma cells leads to a very marked increase in incorporation of radioactivity as well as a significant improvement of cell survival.

AB - Glucose oxidase generates H2O2 via the conversion of glucose to gluconolactone in the presence of molecular oxygen. Coupling of this reaction to the lactoperoxidase-catalyzed substitution of iodine for hydrogen on the phenolic side chains of proteins present on the surfaces of murine myeloma cells leads to a very marked increase in incorporation of radioactivity as well as a significant improvement of cell survival.

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JO - Cellular Immunology

JF - Cellular Immunology

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ER -

The use of glucose oxidase as a generator of H₂O₂ in the enzymatic radioiodination of components of cell surfaces

Abstract

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