The vasodilator 17,18-epoxyeicosatetraenoic acid targets the pore-forming BK α channel subunit in rodents

Hantz C. Hercule, Birgit Salanova, Kirill Essin, Horst Honeck, J R Falck, Matthias Sausbier, Peter Ruth, Wolf Hagen Schunck, Friedrich C. Luft, Maik Gollasch

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

17,18-Epoxyeicosatetraenoic acid (17,18-EETeTr) stimulates vascular large-conductance K+ (BK) channels. BK channels are composed of the pore-forming BK α and auxiliary BK β1 subunits that confer an increased sensitivity for changes in membrane potential and calcium to BK channels. Ryanodine-sensitive calcium-release channels (RyR3) in the sarcoplasmic reticulum (SR) control the process. To elucidate the mechanism of BK channel activation, we performed whole-cell and perforated-patch clamp experiments in freshly isolated cerebral and mesenteric artery vascular smooth muscle cells (VSMC) from Sprague-Dawley rats, BK β1 gene-deficient (-/-), BK α (-/-), RyR3 (-/-) and wild-type mice. The 17,18-EETeTr (100 nm) increased tetraethylammonium (1 mm)-sensitive outward K+ currents in VSMC from wild-type rats and wild-type mice. The effects were not inhibited by the epoxyeicosatrienoic acid (EET) antagonist 14,15-epoxyeicosa-5(Z)-enoic acid (10 μm). BK channel currents were increased 3.5-fold in VSMC from BK β1 (-/-) mice, whereas a 2.9-fold stimulation was observed in VSMC from RyR3 (-/-) mice (at membrane voltage 60 mV). The effects were similar compared with those observed in cells from wild-type mice. The BK current increase was neither influenced by strong internal calcium buffering (Ca2+, 100 nm), nor by external calcium influx. The 17,18-EETeTr did not induce outward currents in VSMC BK α (-/-) cells. We next tested the vasodilator effects of 17,18-EETeTr on isolated arteries of BK α-deficient mice. Vasodilatation was largely inhibited in cerebral and mesenteric arteries isolated from BK α (-/-) mice compared with that observed in wild-type and BK β1 (-/-) arteries. We conclude that 17,18-EETeTr represents an endogenous BK channel agonist and vasodilator. Since 17,18-EETeTr is active in small arteries lacking BK β1, the data further suggest that BK α represents the molecular target for the principal action of 17,18-EETeTr. Finally, the action of 17,18-EETeTr is not mediated by changes of the internal global calcium concentration or local SR calcium release events.

Original languageEnglish (US)
Pages (from-to)1067-1076
Number of pages10
JournalExperimental Physiology
Volume92
Issue number6
DOIs
StatePublished - Nov 2007

ASJC Scopus subject areas

  • Physiology
  • Nutrition and Dietetics
  • Physiology (medical)

Fingerprint Dive into the research topics of 'The vasodilator 17,18-epoxyeicosatetraenoic acid targets the pore-forming BK α channel subunit in rodents'. Together they form a unique fingerprint.

  • Cite this

    Hercule, H. C., Salanova, B., Essin, K., Honeck, H., Falck, J. R., Sausbier, M., Ruth, P., Schunck, W. H., Luft, F. C., & Gollasch, M. (2007). The vasodilator 17,18-epoxyeicosatetraenoic acid targets the pore-forming BK α channel subunit in rodents. Experimental Physiology, 92(6), 1067-1076. https://doi.org/10.1113/expphysiol.2007.038166