The Vi Capsular Polysaccharide Enables Salmonella enterica Serovar Typhi to Evade Microbe-Guided Neutrophil Chemotaxis

Tamding Wangdi, Cheng Yuk Lee, Alanna M. Spees, Chenzhou Yu, Dawn D. Kingsbury, Sebastian E. Winter, Christine J. Hastey, R. Paul Wilson, Volkmar Heinrich, Andreas J. Bäumler

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Salmonella enterica serovar Typhi (S. Typhi) causes typhoid fever, a disseminated infection, while the closely related pathogen S. enterica serovar Typhimurium (S. Typhimurium) is associated with a localized gastroenteritis in humans. Here we investigated whether both pathogens differ in the chemotactic response they induce in neutrophils using a single-cell experimental approach. Surprisingly, neutrophils extended chemotactic pseudopodia toward Escherichia coli and S. Typhimurium, but not toward S. Typhi. Bacterial-guided chemotaxis was dependent on the presence of complement component 5a (C5a) and C5a receptor (C5aR). Deletion of S. Typhi capsule biosynthesis genes markedly enhanced the chemotactic response of neutrophils in vitro. Furthermore, deletion of capsule biosynthesis genes heightened the association of S. Typhi with neutrophils in vivo through a C5aR-dependent mechanism. Collectively, these data suggest that expression of the virulence-associated (Vi) capsular polysaccharide of S. Typhi obstructs bacterial-guided neutrophil chemotaxis.

Original languageEnglish (US)
JournalPLoS pathogens
Volume10
Issue number8
DOIs
StatePublished - 2014

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Molecular Biology
  • Genetics
  • Virology

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    Wangdi, T., Lee, C. Y., Spees, A. M., Yu, C., Kingsbury, D. D., Winter, S. E., Hastey, C. J., Wilson, R. P., Heinrich, V., & Bäumler, A. J. (2014). The Vi Capsular Polysaccharide Enables Salmonella enterica Serovar Typhi to Evade Microbe-Guided Neutrophil Chemotaxis. PLoS pathogens, 10(8). https://doi.org/10.1371/journal.ppat.1004306