The Vi-capsule prevents Toll-like receptor 4 recognition of Salmonella

R. Paul Wilson, Manuela Raffatellu, Daniela Chessa, Sebastian E. Winter, Çagla Tükel, Andreas J. Bäumler

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

The viaB locus enables Salmonella enterica serotype Typhi to reduce Toll-like receptor (TLR) dependent cytokine production in tissue culture models. This DNA region contains genes involved in the regulation (tviA), biosynthesis (tviBCDE) and export (vexABCDE) of the Vi capsule. Expression of the Vi capsule in S. Typhimurium, but not expression of the TviA regulatory protein, reduced tumour necrosis factor-alpha (TNF-α) and IL-6 production by murine bone-marrow derived macrophages. Production of TNF-α and IL-6 was dependent on expression of TLR4 as stimulation of macrophages from TLR4-/- mice with S. Typhimurium did not result in expression of these cytokines. Intraperitoneal infection of mice with S. Typhimurium induced expression of TNF-α and inducible nitric oxide synthase (iNOS) in the liver. Introduction of the cloned viaB region into S. Typhimurium reduced TNF-α and iNOS expression to levels observed after infection with a S. Typhimurium msbB mutant. In contrast, no differences in TNF-α expression between the S. Typhimurium wild type and strains expressing the Vi-capsule or carrying a mutation in msbB were observed after infection of TLR4-/- mice. We conclude that the Vi capsule prevents both in vitro and in vivo recognition of S. Typhimurium lipopolysaccharide by TLR4.

Original languageEnglish (US)
Pages (from-to)876-890
Number of pages15
JournalCellular Microbiology
Volume10
Issue number4
DOIs
StatePublished - Apr 2008

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Toll-Like Receptor 4
Salmonella
Capsules
Tumor Necrosis Factor-alpha
Macrophages
Nitric Oxide Synthase Type II
Interleukin-6
Infection
Cytokines
Tissue culture
Salmonella enterica
Toll-Like Receptors
Biosynthesis
Liver
Lipopolysaccharides
Bone
Genes
Mutation
DNA
Proteins

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Microbiology

Cite this

The Vi-capsule prevents Toll-like receptor 4 recognition of Salmonella. / Wilson, R. Paul; Raffatellu, Manuela; Chessa, Daniela; Winter, Sebastian E.; Tükel, Çagla; Bäumler, Andreas J.

In: Cellular Microbiology, Vol. 10, No. 4, 04.2008, p. 876-890.

Research output: Contribution to journalArticle

Wilson, RP, Raffatellu, M, Chessa, D, Winter, SE, Tükel, Ç & Bäumler, AJ 2008, 'The Vi-capsule prevents Toll-like receptor 4 recognition of Salmonella', Cellular Microbiology, vol. 10, no. 4, pp. 876-890. https://doi.org/10.1111/j.1462-5822.2007.01090.x
Wilson, R. Paul ; Raffatellu, Manuela ; Chessa, Daniela ; Winter, Sebastian E. ; Tükel, Çagla ; Bäumler, Andreas J. / The Vi-capsule prevents Toll-like receptor 4 recognition of Salmonella. In: Cellular Microbiology. 2008 ; Vol. 10, No. 4. pp. 876-890.
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