The Wnt pathway limits BMP signaling outside of the germline stem cell niche in Drosophila ovaries

Violaine I. Mottier-Pavie, Victor Palacios, Susan Eliazer, Shane Scoggin, Michael Buszczak

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The mechanisms that modulate and limit the signaling output of adult stem cell niches remain poorly understood. To gain further insights into how these microenvironments are regulated in vivo, we performed a candidate gene screen designed to identify factors that restrict BMP signal production to the cap cells that comprise the germline stem cell (GSC) niche of Drosophila ovaries. Through these efforts, we found that disruption of Wnt4 and components of the canonical Wnt pathway results in a complex germ cell phenotype marked by an expansion of GSC-like cells, pre-cystoblasts and cystoblasts in young females. This phenotype correlates with an increase of decapentaplegic (dpp) mRNA levels within escort cells and varying levels of BMP responsiveness in the germline. Further genetic experiments show that Wnt4, which exhibits graded expression in somatic cells of germaria, activates the Wnt pathway in posteriorly positioned escort cells. The activation of the Wnt pathway appears to be limited by the BMP pathway itself, as loss of Mad in escort cells results in the expansion of Wnt pathway activation. Wnt pathway activity changes within germaria during the course of aging, coincident with changes in dpp production. These data suggest that mutual antagonism between the BMP and Wnt pathways in somatic cells helps to regulate germ cell differentiation.

Original languageEnglish (US)
Pages (from-to)50-62
Number of pages13
JournalDevelopmental Biology
Volume417
Issue number1
DOIs
StatePublished - Sep 1 2016

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Stem Cell Niche
Wnt Signaling Pathway
Drosophila
Ovary
Germ Cells
Phenotype
Adult Stem Cells
Cell Differentiation
Stem Cells
Messenger RNA

Keywords

  • BMP
  • Germline
  • Niche
  • Stem cells
  • Wnt

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

The Wnt pathway limits BMP signaling outside of the germline stem cell niche in Drosophila ovaries. / Mottier-Pavie, Violaine I.; Palacios, Victor; Eliazer, Susan; Scoggin, Shane; Buszczak, Michael.

In: Developmental Biology, Vol. 417, No. 1, 01.09.2016, p. 50-62.

Research output: Contribution to journalArticle

Mottier-Pavie, Violaine I. ; Palacios, Victor ; Eliazer, Susan ; Scoggin, Shane ; Buszczak, Michael. / The Wnt pathway limits BMP signaling outside of the germline stem cell niche in Drosophila ovaries. In: Developmental Biology. 2016 ; Vol. 417, No. 1. pp. 50-62.
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