The Zn3 domain of human poly(ADP-ribose) polymerase-1 (PARP-1) functions in both DNA-dependent poly(ADP-ribose) synthesis activity and chromatin compaction

Marie France Langelier, Donald D. Ruhl, Jamie L. Planck, W. Lee Kraus, John M. Pascal

Research output: Contribution to journalArticle

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Abstract

PARP-1 is involved in multiple cellular processes, including transcription, DNA repair, and apoptosis. PARP-1 attaches ADP-ribose units to target proteins, including itself as a post-translational modification that can change the biochemical properties of target proteins and mediate recruitment of proteins to sites of poly(ADP-ribose) synthesis. Independent of its catalytic activity, PARP-1 binds to chromatin and promotes compaction affecting RNA polymerase II transcription. PARP-1 has a modular structure composed of six independent domains. Two homologous zinc fingers, Zn1 and Zn2, form the DNA binding module. Zn1-Zn2 binding to DNA breaks triggers catalytic activity. Recently, we have identified a third zinc binding domain in PARP-1, the Zn3 domain, which is essential for DNA dependent PARP-1 activity. The crystal structure of the Zn3 domain revealed a novel zinc-ribbon fold and a homodimeric Zn3 structure that formed in the crystal lattice. Structure guided mutagenesis was used here to investigate the roles of these two features of the Zn3 domain. Our results indicate that the zinc-ribbon fold of the Zn3 domain mediates an interdomain contact crucial to assembly of the DNA-activated conformation of PARP-1. In contrast, residues located at the Zn3 dimer interface are not required for DNA-dependent activation but rather make important contributions to the chromatin compaction activity of PARP-1. Thus, the Zn3 domain has dual roles in regulating the functions of PARP-1.

Original languageEnglish (US)
Pages (from-to)18877-18887
Number of pages11
JournalJournal of Biological Chemistry
Volume285
Issue number24
DOIs
StatePublished - Jun 11 2010

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Poly Adenosine Diphosphate Ribose
Poly(ADP-ribose) Polymerases
Chromatin
Compaction
DNA
Zinc
Transcription
Catalyst activity
Adenosine Diphosphate Ribose
Nucleic Acid Conformation
human PARP1 protein
Poly (ADP-Ribose) Polymerase-1
Mutagenesis
Proteins
DNA Breaks
RNA Polymerase II
Zinc Fingers
Post Translational Protein Processing
Crystal lattices
DNA Repair

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

The Zn3 domain of human poly(ADP-ribose) polymerase-1 (PARP-1) functions in both DNA-dependent poly(ADP-ribose) synthesis activity and chromatin compaction. / Langelier, Marie France; Ruhl, Donald D.; Planck, Jamie L.; Kraus, W. Lee; Pascal, John M.

In: Journal of Biological Chemistry, Vol. 285, No. 24, 11.06.2010, p. 18877-18887.

Research output: Contribution to journalArticle

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