Thermal injury alters endothelial vasoconstrictor and vasodilator response to endotoxin

Joseph T. Murphy, Steven Duffy, Gary F. Purdue, John L. Hunt

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Background: The unique location of the endothelium makes it vulnerable to injury from circulating factors created at remote wounds. In this study, we examined the effect of a sequential burn and lipopolysaccharide (LPS) challenge on endothelial function in vitro. Methods: Human umbilical vein endothelial cells treated with 20% human serum isolated from burn patients (>40% total burn surface area) at 2 and 24 hours postinjury. Cultures were subsequently treated with Escherichia coli LPS:0111:B4 (0.10-100ng/mL). Endothelin-1 (ET-1), 6-ketoPGF(1a), and NO2/NO3 were detected by using specific enzyme immunoassays. Results: Burn serum did not alter endothelial ET-1, PGI2, or NO secretion compared with Control serum. LPS significantly enhanced 6-ketoPGF(1a) (54,242 ± 14,466 pg/106 cells) and NO2/NO3 (723 ± 210 μM) secretion, but not ET-1 compared with Control serum alone (3,878 ± 963 and 219 ± 110). Burn serum pretreatment significantly enhanced the ET-1 response to LPS (303 ± 36 pg/106 cells vs. 193 ± 47). The 6-ketoPGF(1a) (16,509 ± 3,785) and NO2/NO3 (354 ± 98) responses to Burn/LPS were significantly diminished compared with Control/LPS. Although this level of 6- ketoPGF(1a) was elevated compared with Control alone (7,518 ± 2,299), NO2/NO3 was unchanged (significance at p < 0.05). Conclusion: Thermal injury may prime remote endothelium and alter the response to a septic focus with an enhanced vasoconstrictor (ET-1) and diminished vasodilator (PGI2/NO) response, a situation that may contribute to postburn distal organ injury.

Original languageEnglish (US)
Pages (from-to)492-499
Number of pages8
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume47
Issue number3
DOIs
StatePublished - Sep 1 1999

Keywords

  • Endothelial cell
  • Endotoxin
  • Permeability
  • Thermal injury
  • Vasoactivity

ASJC Scopus subject areas

  • Surgery
  • Critical Care and Intensive Care Medicine

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