Three cryptochromes are rhythmically expressed in Xenopus laevis retinal photoreceptors

Haisun Zhu, Carla B. Green

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Purpose: To clone Xenopus laevis cryptochromes (crys) and to understand their role in the Xenopus retinal clock. Methods: We designed degenerate PCR primers based on homology between mouse and human crys. DNA fragments generated from these PCR reactions were used to screen a Xenopus retinal cDNA library. Three independent clones were identified and sequenced. The temporal and spatial expression of these genes in retina were studied by Northern blot analysis and in situ hybridization. Results: We cloned three cry homologs from Xenopus laevis retina. We named them xcry1, xcry2a, and xcry2b based on their high homology to the mouse crys. Sequence analysis shows that these Xenopus CRYs have more than 85% identity to mouse CRYs at the amino acid level. Northern blot analysis demonstrated that all three xcrys are rhythmically expressed in the retina with peaks at different times of the day. The xcrys are expressed in a variety of tissues. In retina, they are expressed predominantly in photoreceptor cells. Conclusions: Our finding of cry expression in Xenopus photoreceptor cells further supports the idea of independent circadian oscillators being present in these cells. The sequence similarities to mouse crys suggest similar functions in the circadian clock. However, their distinct temporal expression patterns suggest some unique role for xCRY in the Xenopus retina.

Original languageEnglish (US)
Pages (from-to)210-215
Number of pages6
JournalMolecular Vision
Volume7
StatePublished - 2001

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Cryptochromes
Vertebrate Photoreceptor Cells
Xenopus laevis
Xenopus
Retina
Photoreceptor Cells
Northern Blotting
Clone Cells
Polymerase Chain Reaction
Circadian Clocks
Gene Library
In Situ Hybridization
Sequence Analysis
Gene Expression
Amino Acids
DNA

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Three cryptochromes are rhythmically expressed in Xenopus laevis retinal photoreceptors. / Zhu, Haisun; Green, Carla B.

In: Molecular Vision, Vol. 7, 2001, p. 210-215.

Research output: Contribution to journalArticle

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