@inproceedings{e5de57a14e5b45969b63e817fce9b2ad,
title = "Three-dimensional deep tissue multiphoton frequency-domain fluorescence lifetime imaging microscopy via phase multiplexing and adaptive optics",
abstract = "We propose and demonstrate a novel multiphoton frequency-domain fluorescence lifetime imaging microscopy (MPM-FD-FLIM) system that is able to generate 3D lifetime images in deep scattering tissues. The imaging speed of FD-FLIM is improved using phase multiplexing, where the fluorescence signal is split and mixed with the reference signal from the laser in a multiplexing manner. The system allows for easy generation of phasor plots, which not only address multi-exponential decay problems but also clearly represent the dynamics of the fluorophores being investigated. Lastly, a sensorless adaptive optics setup is used for FLIM imaging in deep scattering tissues. The capability of the system is demonstrated in fixed and living animal models, including mice and zebrafish.",
keywords = "Adaptive optics, Deep tissue imaging, Fluorescence lifetime imaging microscopy, Frequency-domain, In vivo imaging, Multiphoton microscopy, Multiplexing, Radio frequency",
author = "Yide Zhang and Guldner, {Ian H.} and Nichols, {Evan L.} and David Benirschke and Smith, {Cody J.} and Siyuan Zhang and Howard, {Scott S.}",
note = "Publisher Copyright: {\textcopyright} COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.; Multiphoton Microscopy in the Biomedical Sciences XIX 2019 ; Conference date: 03-02-2019 Through 06-02-2019",
year = "2019",
doi = "10.1117/12.2510674",
language = "English (US)",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Ammasi Periasamy and So, {Peter T. C.} and Karsten Konig",
booktitle = "Multiphoton Microscopy in the Biomedical Sciences XIX",
}