Three-dimensional imaging of corneal cells using in vivo confocal microscopy

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Confocal microscopy is a unique and powerful imaging paradigm which allows optical sectioning through intact tissue. Real-time tandem scanning confocal micrsocopy has previously been used to generate high-magnification two-dimensional (2-D) images of cells in living organ systems. Inherent problems with movement, however, have prevented the in vivo acquisition of complete 3-D datasets. The development of a new objective lens, used in combination with specialized real-time imaging acquisition procedures, has allowed sequential serial sections to be obtained in vivo from the rabbit cornea for the first time. These sections can be digitally registered and stacked on the computer to provide a 3-D reconstruction of the corneal cells. This technique should serve as a useful method for studying 3-D structures and analysing 4-D phenomenon at the cellular level in living animals. Three-dimensional images of a stromal nerve in normal rabbit corneal wound are presented as examples of current capabilities.

Original languageEnglish (US)
Pages (from-to)213-219
Number of pages7
JournalJournal of Microscopy
Volume170
Issue number3
StatePublished - 1993

Fingerprint

Three-Dimensional Imaging
Confocal microscopy
rabbits
Confocal Microscopy
acquisition
Rabbits
microscopy
Imaging techniques
cornea
nerves
magnification
cells
organs
Cornea
Lenses
animals
Animals
lenses
Tissue
Scanning

Keywords

  • Confocal microscopy
  • Cornea
  • Digital imaging
  • Eye
  • In vivo
  • Reconstruction
  • Three-dimensional

ASJC Scopus subject areas

  • Instrumentation

Cite this

Three-dimensional imaging of corneal cells using in vivo confocal microscopy. / Petroll, Walter M; Cavanagh, Harrison D; Jester, J. V.

In: Journal of Microscopy, Vol. 170, No. 3, 1993, p. 213-219.

Research output: Contribution to journalArticle

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