Three-dimensional in vivo near-infrared photoacoustic tomography of whole small animal head

Kwang Hyun Song, George Stoica, Lihong V. Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

A three-dimensional in vivo near-infrared photoacoustic tomography imaging system was newly designed and built to visualize the structure of a whole small animal head. For high sensitivity, a single flat 2.25MHz low frequency transducer, whose active element size is 6mm, was employed. To increase the penetration depth of light, a wavelength of 804nm in the NIR range, which matches the oxy- and deoxy-hemoglobin isosbestic point, was chosen. To avoid strong photoacoustic signal generation from the skin surface, we applied dark field illumination. To illuminate efficiently, we split the laser light into two beams, which were delivered to an animal by two mirrors and were finally homogenized by two ground glasses. To complete the dark field illumination, the transducer was located in the middle of two light sources. Two key devices for the in vivo imaging were rotating devices and animal holders. The rotating devices were composed of two parts, located at the top and bottom, which rotated at the same angular speed. The holders were composed of a head holder and a body holder. Both holders fixed the animal firmly to reduce motion artifacts. This system achieved radial resolution of up to 260μm. We accomplished successful in vivo imaging of arterial and venous vessels deeply, as well as superficially, with the animal head of up to 1.7cm diameter. The technique forms a basis for functional imaging, such as measurement of the oxygen consumption ratio in the brain, which is a vital parameter in a brain disease research.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
DOIs
StatePublished - May 8 2006
Event7th Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics - Photons Plus Ultrasound: Imaging and Sensing 2006 - San Jose, CA, United States
Duration: Jan 22 2006Jan 26 2006

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume6086
ISSN (Print)0277-786X

Other

Other7th Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics - Photons Plus Ultrasound: Imaging and Sensing 2006
CountryUnited States
CitySan Jose, CA
Period1/22/061/26/06

Keywords

  • In vivo
  • Mouse holder
  • Photoacoustic
  • Rotating devices
  • Three-dimensional

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Song, K. H., Stoica, G., & Wang, L. V. (2006). Three-dimensional in vivo near-infrared photoacoustic tomography of whole small animal head. In Proceedings of SPIE - The International Society for Optical Engineering [60860Q] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 6086). https://doi.org/10.1117/12.646681