Three-dimensional spheroid culture targeting versatile tissue bioassays using a PDMS-based hanging drop array

Ching Te Kuo, Jong Yueh Wang, Yu Fen Lin, Andrew M. Wo, Benjamin P.C. Chen, Hsinyu Lee

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Biomaterial-based tissue culture platforms have emerged as useful tools to mimic in vivo physiological microenvironments in experimental cell biology and clinical studies. We describe herein a three-dimensional (3D) tissue culture platform using a polydimethylsiloxane (PDMS)-based hanging drop array (PDMS-HDA) methodology. Multicellular spheroids can be achieved within 24 h and further boosted by incorporating collagen fibrils in PDMS-HDA. In addition, the spheroids generated from different human tumor cells exhibited distinct sensitivities toward drug chemotherapeutic agents and radiation as compared with two-dimensional (2D) cultures that often lack in vivo-like biological insights. We also demonstrated that multicellular spheroids may enable key hallmarks of tissue-based bioassays, including drug screening, tumor dissemination, cell co-culture, and tumor invasion. Taken together, these results offer new opportunities not only to achieve the active control of 3D multicellular spheroids on demand, but also to establish a rapid and cost-effective platform to study anti-cancer therapeutics and tumor microenvironments.

Original languageEnglish (US)
Article number4718
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

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Cellular Spheroids
Biological Assay
Tumor Microenvironment
Neoplasms
Preclinical Drug Evaluations
Biocompatible Materials
Coculture Techniques
Cell Biology
Collagen
Cell Culture Techniques
Radiation
Costs and Cost Analysis
Pharmaceutical Preparations
baysilon
Therapeutics

ASJC Scopus subject areas

  • General

Cite this

Three-dimensional spheroid culture targeting versatile tissue bioassays using a PDMS-based hanging drop array. / Kuo, Ching Te; Wang, Jong Yueh; Lin, Yu Fen; Wo, Andrew M.; Chen, Benjamin P.C.; Lee, Hsinyu.

In: Scientific Reports, Vol. 7, No. 1, 4718, 01.12.2017.

Research output: Contribution to journalArticle

Kuo, Ching Te ; Wang, Jong Yueh ; Lin, Yu Fen ; Wo, Andrew M. ; Chen, Benjamin P.C. ; Lee, Hsinyu. / Three-dimensional spheroid culture targeting versatile tissue bioassays using a PDMS-based hanging drop array. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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