Three-dimensional structure of the ultraoligotrophic marine bacterium "Candidatus pelagibacter ubique"

Xiaowei Zhao, Cindi L. Schwartz, Jason Pierson, Stephen J. Giovannoni, J. Richard McIntosh, Daniela Nicastro

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

SAR11 bacteria are small, heterotrophic, marine alphaproteobacteria found throughout the oceans. They thrive at the low nutrient concentrations typical of open ocean conditions, although the adaptations required for life under those conditions are not well understood. To illuminate this issue, we used cryo-electron tomography to study "Candidatus Pelagibacter ubique" strain HTCC1062, a member of the SAR11 clade. Our results revealed its cellular dimensions and details of its intracellular organization. Frozen-hydrated cells, which were preserved in a life-like state, had an average cell volume (enclosed by the outer membrane) of 0.037 ± 0.011 μm3. Strikingly, the periplasmic space occupied ~20% to 50% of the total cell volume in log-phase cells and ~50% to 70% in stationary-phase cells. The nucleoid occupied the convex side of the crescent-shaped cells and the ribosomes predominantly occupied the concave side, at a relatively high concentration of 10,000 to 12,000 ribosomes/μm3. Outer membrane pore complexes, likely composed of PilQ, were frequently observed in both log-phase and stationary-phase cells. Long filaments, most likely type IV pili, were found on dividing cells. The physical dimensions, intracellular organization, and morphological changes throughout the life cycle of "Ca. Pelagibacter ubique" provide structural insights into the functional adaptions of these oligotrophic ultramicrobacteria to their habitat.

Original languageEnglish (US)
Article numbere02807-16
JournalApplied and Environmental Microbiology
Volume83
Issue number3
DOIs
StatePublished - Jan 1 2017

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membrane
Bacteria
bacterium
bacteria
open ocean
tomography
life cycle
cells
Ribosomes
Cell Size
Oceans and Seas
electron
nutrient
ocean
habitat
ribosomes
Electron Microscope Tomography
Alphaproteobacteria
Periplasm
Membranes

Keywords

  • Cryo-electron tomography
  • PilQ
  • SAR11
  • Type IV pili
  • Ultramicrobacteria

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Three-dimensional structure of the ultraoligotrophic marine bacterium "Candidatus pelagibacter ubique". / Zhao, Xiaowei; Schwartz, Cindi L.; Pierson, Jason; Giovannoni, Stephen J.; McIntosh, J. Richard; Nicastro, Daniela.

In: Applied and Environmental Microbiology, Vol. 83, No. 3, e02807-16, 01.01.2017.

Research output: Contribution to journalArticle

Zhao, Xiaowei ; Schwartz, Cindi L. ; Pierson, Jason ; Giovannoni, Stephen J. ; McIntosh, J. Richard ; Nicastro, Daniela. / Three-dimensional structure of the ultraoligotrophic marine bacterium "Candidatus pelagibacter ubique". In: Applied and Environmental Microbiology. 2017 ; Vol. 83, No. 3.
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