Abstract
We isolated from a HeLa genomic library 38 plaques that hybridized to total mitochondrial (mt) DNA isolated from human placenta. One clone (HLmt-17·8) hybridized to a 740 base-pair (12 S ribosomal RNA gene and displacement loop) mtDNA probe and was characterized in more detail. Within its 17·8 × 103 base-pair insert a 1·6 × 103 base-pair mtDNA fragment was similar to three non-sequential coding genes of human mtDNA, including a part of the 12 S ribosomal RNA (684-971), the cytochrome oxidase I (6553-7302), and two NADH dehydrogenase [ ND4L ND4] (10,606-11,159). The similarity to human mtDNA sequences was 92·0%, 92·3% and 92·4%, respectively, the highest degree of similarity to human mtDNA so far reported. This is also the first report of several adjacent mtDNA-like sequences in cellular chromosomes. The mtDNA-like sequences in HLmt-17·8 was found in the DNAs of human placenta, freshly isolated human leukocytes, foreskin and several human cell lines; but it was not present in other primates or lower organisms. The HLmt-17.8 mtDNA-like region appears to be a pseudogene that transferred into the nucleus in humans more recently than nine million years ago.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 703-707 |
| Number of pages | 5 |
| Journal | Journal of Molecular Biology |
| Volume | 210 |
| Issue number | 4 |
| DOIs | |
| State | Published - Dec 20 1989 |
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ASJC Scopus subject areas
- Virology
Cite this
Three separate mitochondrial DNA sequences are contiguous in human genomic DNA. / Kamimura, Naohisa; Ishii, Shougo; Liandong, Ma; Shay, Jerry W.
In: Journal of Molecular Biology, Vol. 210, No. 4, 20.12.1989, p. 703-707.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Three separate mitochondrial DNA sequences are contiguous in human genomic DNA
AU - Kamimura, Naohisa
AU - Ishii, Shougo
AU - Liandong, Ma
AU - Shay, Jerry W.
PY - 1989/12/20
Y1 - 1989/12/20
N2 - We isolated from a HeLa genomic library 38 plaques that hybridized to total mitochondrial (mt) DNA isolated from human placenta. One clone (HLmt-17·8) hybridized to a 740 base-pair (12 S ribosomal RNA gene and displacement loop) mtDNA probe and was characterized in more detail. Within its 17·8 × 103 base-pair insert a 1·6 × 103 base-pair mtDNA fragment was similar to three non-sequential coding genes of human mtDNA, including a part of the 12 S ribosomal RNA (684-971), the cytochrome oxidase I (6553-7302), and two NADH dehydrogenase [ ND4L ND4] (10,606-11,159). The similarity to human mtDNA sequences was 92·0%, 92·3% and 92·4%, respectively, the highest degree of similarity to human mtDNA so far reported. This is also the first report of several adjacent mtDNA-like sequences in cellular chromosomes. The mtDNA-like sequences in HLmt-17·8 was found in the DNAs of human placenta, freshly isolated human leukocytes, foreskin and several human cell lines; but it was not present in other primates or lower organisms. The HLmt-17.8 mtDNA-like region appears to be a pseudogene that transferred into the nucleus in humans more recently than nine million years ago.
AB - We isolated from a HeLa genomic library 38 plaques that hybridized to total mitochondrial (mt) DNA isolated from human placenta. One clone (HLmt-17·8) hybridized to a 740 base-pair (12 S ribosomal RNA gene and displacement loop) mtDNA probe and was characterized in more detail. Within its 17·8 × 103 base-pair insert a 1·6 × 103 base-pair mtDNA fragment was similar to three non-sequential coding genes of human mtDNA, including a part of the 12 S ribosomal RNA (684-971), the cytochrome oxidase I (6553-7302), and two NADH dehydrogenase [ ND4L ND4] (10,606-11,159). The similarity to human mtDNA sequences was 92·0%, 92·3% and 92·4%, respectively, the highest degree of similarity to human mtDNA so far reported. This is also the first report of several adjacent mtDNA-like sequences in cellular chromosomes. The mtDNA-like sequences in HLmt-17·8 was found in the DNAs of human placenta, freshly isolated human leukocytes, foreskin and several human cell lines; but it was not present in other primates or lower organisms. The HLmt-17.8 mtDNA-like region appears to be a pseudogene that transferred into the nucleus in humans more recently than nine million years ago.
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U2 - 10.1016/0022-2836(89)90103-4
DO - 10.1016/0022-2836(89)90103-4
M3 - Article
VL - 210
SP - 703
EP - 707
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
SN - 0022-2836
IS - 4
ER -