Three separate mitochondrial DNA sequences are contiguous in human genomic DNA

Naohisa Kamimura, Shougo Ishii, Ma Liandong, Jerry W. Shay

Research output: Contribution to journalArticle

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Abstract

We isolated from a HeLa genomic library 38 plaques that hybridized to total mitochondrial (mt) DNA isolated from human placenta. One clone (HLmt-17·8) hybridized to a 740 base-pair (12 S ribosomal RNA gene and displacement loop) mtDNA probe and was characterized in more detail. Within its 17·8 × 103 base-pair insert a 1·6 × 103 base-pair mtDNA fragment was similar to three non-sequential coding genes of human mtDNA, including a part of the 12 S ribosomal RNA (684-971), the cytochrome oxidase I (6553-7302), and two NADH dehydrogenase [ ND4L ND4] (10,606-11,159). The similarity to human mtDNA sequences was 92·0%, 92·3% and 92·4%, respectively, the highest degree of similarity to human mtDNA so far reported. This is also the first report of several adjacent mtDNA-like sequences in cellular chromosomes. The mtDNA-like sequences in HLmt-17·8 was found in the DNAs of human placenta, freshly isolated human leukocytes, foreskin and several human cell lines; but it was not present in other primates or lower organisms. The HLmt-17.8 mtDNA-like region appears to be a pseudogene that transferred into the nucleus in humans more recently than nine million years ago.

Original languageEnglish (US)
Pages (from-to)703-707
Number of pages5
JournalJournal of Molecular Biology
Volume210
Issue number4
DOIs
StatePublished - Dec 20 1989

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Mitochondrial DNA
DNA
Base Pairing
Placenta
Foreskin
NADH Dehydrogenase
Pseudogenes
Ribosomal RNA
Genomic Library
Electron Transport Complex IV
rRNA Genes
Primates
Leukocytes
Clone Cells
Chromosomes
Cell Line
Genes

ASJC Scopus subject areas

  • Virology

Cite this

Three separate mitochondrial DNA sequences are contiguous in human genomic DNA. / Kamimura, Naohisa; Ishii, Shougo; Liandong, Ma; Shay, Jerry W.

In: Journal of Molecular Biology, Vol. 210, No. 4, 20.12.1989, p. 703-707.

Research output: Contribution to journalArticle

Kamimura, Naohisa ; Ishii, Shougo ; Liandong, Ma ; Shay, Jerry W. / Three separate mitochondrial DNA sequences are contiguous in human genomic DNA. In: Journal of Molecular Biology. 1989 ; Vol. 210, No. 4. pp. 703-707.
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