Three separate mitochondrial DNA sequences are contiguous in human genomic DNA

Naohisa Kamimura, Shougo Ishii, Ma Liandong, Jerry W. Shay

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

We isolated from a HeLa genomic library 38 plaques that hybridized to total mitochondrial (mt) DNA isolated from human placenta. One clone (HLmt-17·8) hybridized to a 740 base-pair (12 S ribosomal RNA gene and displacement loop) mtDNA probe and was characterized in more detail. Within its 17·8 × 103 base-pair insert a 1·6 × 103 base-pair mtDNA fragment was similar to three non-sequential coding genes of human mtDNA, including a part of the 12 S ribosomal RNA (684-971), the cytochrome oxidase I (6553-7302), and two NADH dehydrogenase [ ND4L ND4] (10,606-11,159). The similarity to human mtDNA sequences was 92·0%, 92·3% and 92·4%, respectively, the highest degree of similarity to human mtDNA so far reported. This is also the first report of several adjacent mtDNA-like sequences in cellular chromosomes. The mtDNA-like sequences in HLmt-17·8 was found in the DNAs of human placenta, freshly isolated human leukocytes, foreskin and several human cell lines; but it was not present in other primates or lower organisms. The HLmt-17.8 mtDNA-like region appears to be a pseudogene that transferred into the nucleus in humans more recently than nine million years ago.

Original languageEnglish (US)
Pages (from-to)703-707
Number of pages5
JournalJournal of Molecular Biology
Volume210
Issue number4
DOIs
StatePublished - Dec 20 1989

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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