Thrombin-mediated permeability of human microvascular pulmonary endothelial cells is calcium dependent

Joseph T. Murphy, Steve L. Duffy, Dixie L. Hybki, Kristine Kamm

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background: In response to inflammation, endothelial cytoskeleton rearrangement, cell contraction, and intercellular gap formation contribute to a loss of capillary barrier integrity and resultant interstitial edema formation. The intracellular signals controlling these events are thought to be dependent on intracellular calcium concentration ([Ca2+]i). We hypothesized that, in human pulmonary microvascular endothelial cells, a thrombin-induced increase in permeability to albumin would be dependent on [Ca2+]i and subsequent actin cytoskeleton rearrangements. Methods: Human lung microvascular endothelial cells, grown on 0.4 μmol/L pore membranes, were activated with 10 nmol/L human thrombin in Hank's balanced salt solution/0.5% fetal bovine serum. Select cultures were pretreated (45 minutes) with 4 μmol Fura-2/AM to chelate Ca2+ i. Permeability was assessed as diffusion of bovine serum albumin/biotin across the monolayer. Similarly treated cells were stained with rhodamine-phalloidin to demonstrate actin cytoskeletal morphology. Separately, cells loaded 2 μmol Fura-2/AM were assessed at OD340/380nm after thrombin exposure to detect free [Ca2+]i. Results: Intracellular [Ca2+] levels increased 15-fold (2 seconds) and fell to baseline (10 minutes) after thrombin. Permeability increased 10-fold (30 minutes), and a shift from cortical to actin stress fiber morphology was observed. Chelation of Ca2+ i diminished permeability to baseline and reduced the percentage of cells exhibiting stress fiber formation. Conclusion: Thrombin stimulates pulmonary capillary leak by affecting the barrier function of activated pulmonary endothelial cells. These data demonstrate a thrombin-stimulated increase in monolayer permeability, and cytoskeletal F-actin stress fibers were, in part, regulated by endothelial [Ca2+]i. This early, transient rise in [Ca2+]i likely activates downstream pathways that more directly affect the intracellular endothelial structural changes that control vascular integrity.

Original languageEnglish (US)
Pages (from-to)213-222
Number of pages10
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume50
Issue number2
StatePublished - 2001

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Capillary Permeability
Thrombin
Endothelial Cells
Permeability
Calcium
Lung
Stress Fibers
Actins
Fura-2
Biotin
Bovine Serum Albumin
Cytoskeleton
Actin Cytoskeleton
Blood Vessels
Albumins
Edema
Salts
Inflammation
Membranes
Serum

ASJC Scopus subject areas

  • Surgery

Cite this

Thrombin-mediated permeability of human microvascular pulmonary endothelial cells is calcium dependent. / Murphy, Joseph T.; Duffy, Steve L.; Hybki, Dixie L.; Kamm, Kristine.

In: Journal of Trauma - Injury, Infection and Critical Care, Vol. 50, No. 2, 2001, p. 213-222.

Research output: Contribution to journalArticle

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