TY - JOUR
T1 - Thy-1 antigen-bearing dendritic cells in murine epidermis are derived from bone marrow precursors
AU - Bergstresser, P. R.
AU - Tigelaar, R. E.
AU - Streilein, J. W.
N1 - Funding Information:
Cells were identified by immunofluorescence microscopy in whole mounl S of epidermis as described previously 11 ,7 1. Bri efl y, epidermis was separal ed from dermis a i't er incubal ion for 4 h ina bu ffered medium Manuscripl received .January ] 0, 1984: accepted for publication March 15, 198,1 Supported in part by U.S. Public Healt.h Service Grants AI-17363 a nd AM-28425. Reprinl requesls 1.0: Dr. Paul R. Bergstresser, Department of Dermatology, Un iversil Y of T exas Heal! h Science Cent er at Dallas, 5:123 Harry Hines Boulevard, Dallas, Texas 75235. Abb reviat ions: EDTA: el hylenediaillinetel macetic acid LCs: Langerhans cells PBS: phosphate·hutTered saline T hy· ] +: Thy-I a nI ige n-bearing cell s
PY - 1984
Y1 - 1984
N2 - Thy-1 antigen is expressed on a dendritic subpopulation of cells in murine epidermis. Numbering between 200 and 500/mm2 surface area in abdominal skin, they are distinct from the dendritic Langerhans cells (LCs) and melanocytes. Since immigrant lymphoid cells as well as constructive cells in various organs have been demonstrated to be Thy-1+, their origin and function are not certain. To assess these issues, two experimental protocols were established. First, grafts of whole skin from AKR mice were placed orthotopically on (AKD2)F1 recipients. Immigration of recipient-derived cells into graft epidermis was assessed histologically by fluorescence microscopy employing monoclonal anti-Thy-1.2 and anti-I-A(d) antibodies. Second, bone marrow chimeras were established in AKR recipients after lethal irradiation and reconstitution with cells from (AKD2)F1 donors. In the first protocol, dendritic I-A(d+) LCs of donor origin infiltrated each graft to normal densities within 2 weeks. Thy-1.2+ cells also immigrated into the same grafts, but at much slower rates. In the second protocol, bone marrow-derived Thy-1.2+ cells populated normal skin epidermis slowly over several months, with densities reaching 70/mm2. We conclude that some, if not all, Thy-1+ cells in normal murine epidermis are derived from bone marrow precursors, that their infiltration rates differ substantially from those of LCs, and that those factors which govern immigration ratios into adult skin derive from the skin itself rather than from the systemic availability of their precursors. We suggest that the function of Thy-1+ epidermal cells will therefore reside among those usually ascribed to recirculating hematogenous cells, including the possibility thay they may down-regulate immunizing signals that emerge from skin.
AB - Thy-1 antigen is expressed on a dendritic subpopulation of cells in murine epidermis. Numbering between 200 and 500/mm2 surface area in abdominal skin, they are distinct from the dendritic Langerhans cells (LCs) and melanocytes. Since immigrant lymphoid cells as well as constructive cells in various organs have been demonstrated to be Thy-1+, their origin and function are not certain. To assess these issues, two experimental protocols were established. First, grafts of whole skin from AKR mice were placed orthotopically on (AKD2)F1 recipients. Immigration of recipient-derived cells into graft epidermis was assessed histologically by fluorescence microscopy employing monoclonal anti-Thy-1.2 and anti-I-A(d) antibodies. Second, bone marrow chimeras were established in AKR recipients after lethal irradiation and reconstitution with cells from (AKD2)F1 donors. In the first protocol, dendritic I-A(d+) LCs of donor origin infiltrated each graft to normal densities within 2 weeks. Thy-1.2+ cells also immigrated into the same grafts, but at much slower rates. In the second protocol, bone marrow-derived Thy-1.2+ cells populated normal skin epidermis slowly over several months, with densities reaching 70/mm2. We conclude that some, if not all, Thy-1+ cells in normal murine epidermis are derived from bone marrow precursors, that their infiltration rates differ substantially from those of LCs, and that those factors which govern immigration ratios into adult skin derive from the skin itself rather than from the systemic availability of their precursors. We suggest that the function of Thy-1+ epidermal cells will therefore reside among those usually ascribed to recirculating hematogenous cells, including the possibility thay they may down-regulate immunizing signals that emerge from skin.
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U2 - 10.1111/1523-1747.ep12262587
DO - 10.1111/1523-1747.ep12262587
M3 - Article
C2 - 6147381
AN - SCOPUS:0021213711
SN - 0022-202X
VL - 83
SP - 83
EP - 87
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 2
ER -