Thymidine kinase 2 (H126N) knockin mice show the essential role of balanced deoxynucleotide pools for mitochondrial DNA maintenance

Hasan O. Akman, Beatriz Dorado, Luis C. López, Ángeles García-Cazorla, Maya R. Vilà, Lauren M. Tanabe, William T. Dauer, Eduardo Bonilla, Kurenai Tanji, Michio Hirano

Research output: Contribution to journalArticlepeer-review

94 Scopus citations

Abstract

Mitochondrial DNA (mtDNA) depletion syndrome (MDS), an autosomal recessive condition, is characterized by variable organ involvement with decreased mtDNA copy number and activities of respiratory chain enzymes in affected tissues. MtDNA depletion has been associated with mutations in nine autosomal genes, including thymidine kinase (TK2), which encodes a ubiquitous mitochondrial protein. To study the pathogenesis of TK2-deficiency, we generated mice harboring an H126N Tk2 mutation. Homozygous Tk2 mutant (Tk2-/-) mice developed rapidly progressive weakness after age 10 days and died between ages 2 and 3 weeks. Tk2-/- animals showed Tk2 deficiency, unbalanced dNTP pools, mtDNA depletion and defects of respiratory chain enzymes containing mtDNA-encoded subunits that were most prominent in the central nervous system. Histopathology revealed an encephalomyelopathy with prominent vacuolar changes in the anterior horn of the spinal cord. The H126N TK2 mouse is the first knock-in animal model of human MDS and demonstrates that the severity of TK2 deficiency in tissues may determine the organ-specific phenotype. The Author 2008. Published by Oxford University Press. All rights reserved.

Original languageEnglish (US)
Pages (from-to)2433-2440
Number of pages8
JournalHuman molecular genetics
Volume17
Issue number16
DOIs
StatePublished - Aug 2008
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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