Thymosin β10 and thymosin β4 are both actin monomer sequestering proteins

Fu Xin Yu, Sheng Cai Lin, Marcelle Morrison-Bogorad, Mark A L Atkinson, Helen L. Yin

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Abstract

The β-thymosins are a family of related peptides. Recently, thymosin β4 was identified as a significant actin monomer sequestering protein in cells. To determine if other β-thymosins also bind actin, and how they may participate in the regulation of actin polymerization, we expressed thymosin β4 and its major homolog, thymosin β10, in bacteria and characterized their interactions with actin. Equilibrium sedimentation studies showed that thymosin β4 behaved as a monomeric protein in solution. Both β-thymosins bound skeletal muscle actin and inhibited actin polymerization with similar Kd values (between 0.7-1 μM). They were not inhibited by polyphosphoinositides. Kinetic measurements showed that at high ratios of β-thymosin to actin, β-thymosin decreased the rate of barbed end filament growth. However, in spite of a close agreement between the kinetic and steady state Kd values, the rate of barbed end filament growth was slightly, but reproducibly, larger than expected, and this deviation was particularly noticeable at lower ratios of β-thymosin to actin. We conclude that unlike profilin, β-thymosins are primarily actin monomer sequestering proteins, although some aspects of their interactions with actin are still not completely understood.

Original languageEnglish (US)
Pages (from-to)502-509
Number of pages8
JournalJournal of Biological Chemistry
Volume268
Issue number1
StatePublished - Jan 5 1993

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Thymosin
Actins
Monomers
Proteins
Polymerization
Profilins
Phosphatidylinositol Phosphates
Kinetics
Growth
Sedimentation
Muscle
Bacteria
Skeletal Muscle

ASJC Scopus subject areas

  • Biochemistry

Cite this

Yu, F. X., Lin, S. C., Morrison-Bogorad, M., Atkinson, M. A. L., & Yin, H. L. (1993). Thymosin β10 and thymosin β4 are both actin monomer sequestering proteins. Journal of Biological Chemistry, 268(1), 502-509.

Thymosin β10 and thymosin β4 are both actin monomer sequestering proteins. / Yu, Fu Xin; Lin, Sheng Cai; Morrison-Bogorad, Marcelle; Atkinson, Mark A L; Yin, Helen L.

In: Journal of Biological Chemistry, Vol. 268, No. 1, 05.01.1993, p. 502-509.

Research output: Contribution to journalArticle

Yu, FX, Lin, SC, Morrison-Bogorad, M, Atkinson, MAL & Yin, HL 1993, 'Thymosin β10 and thymosin β4 are both actin monomer sequestering proteins', Journal of Biological Chemistry, vol. 268, no. 1, pp. 502-509.
Yu FX, Lin SC, Morrison-Bogorad M, Atkinson MAL, Yin HL. Thymosin β10 and thymosin β4 are both actin monomer sequestering proteins. Journal of Biological Chemistry. 1993 Jan 5;268(1):502-509.
Yu, Fu Xin ; Lin, Sheng Cai ; Morrison-Bogorad, Marcelle ; Atkinson, Mark A L ; Yin, Helen L. / Thymosin β10 and thymosin β4 are both actin monomer sequestering proteins. In: Journal of Biological Chemistry. 1993 ; Vol. 268, No. 1. pp. 502-509.
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