Thymus-dependent memory phenotype CD8 T cells in naive B6.H-2K b-/-Db-/- animals mediate an antigen-specific response against Listeria monocytogenes

Jie Su, Rance E. Berg, Sean Murray, James Forman

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

B6.H-2Kb-/-Db-/- (DKO) mice have greatly reduced numbers of mature CD8αβ T cells in their periphery. However, these non-class Ia-selected CD8αβ T cells are able to mediate immune responses to a number of pathogens. Approximately 60% of the CD8αβ T cells in the spleen and peripheral lymph nodes of naive DKO mice display a memory (CD44 high) phenotype. To investigate the origins of these non-class Ia-selected CD8αβCD44high cells, we traced the phenotype of recent thymic emigrants and found that most were CD44low. We also determined whether their appearance was thymus dependent and found that only a small percentage of non-class Ia-selected CD8αβCD44high cells develop in a thymus-independent pathway. Functionally, CD8αβCD44high cells from DKO mice are able to secrete IFN-γ in response to IL-12 and IL-18 in the absence of cognate Ag. When challenged with anti-CD3 in vivo, nearly half of these cells produce IFN-γ within 3 h. When panned CD8αβCD44high cells from Thy1.2.DKO mice were transferred into Thy1.1 DKO recipients and then challenged with Listeria monocytogenes, an Ag-specific anti-L. monocytogenes response was observed 6 days later. Our data suggest that non-class Ia-selected CD8αβCD44high cells in naive animals can respond rapidly to Ag and play a role in the innate as well as the early phase of the acquired immune response.

Original languageEnglish (US)
Pages (from-to)6450-6457
Number of pages8
JournalJournal of Immunology
Volume175
Issue number10
StatePublished - Nov 15 2005

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Listeria monocytogenes
Thymus Gland
T-Lymphocytes
Phenotype
Antigens
Interleukin-18
Interleukin-12
Spleen
Lymph Nodes

ASJC Scopus subject areas

  • Immunology

Cite this

Thymus-dependent memory phenotype CD8 T cells in naive B6.H-2K b-/-Db-/- animals mediate an antigen-specific response against Listeria monocytogenes. / Su, Jie; Berg, Rance E.; Murray, Sean; Forman, James.

In: Journal of Immunology, Vol. 175, No. 10, 15.11.2005, p. 6450-6457.

Research output: Contribution to journalArticle

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abstract = "B6.H-2Kb-/-Db-/- (DKO) mice have greatly reduced numbers of mature CD8αβ T cells in their periphery. However, these non-class Ia-selected CD8αβ T cells are able to mediate immune responses to a number of pathogens. Approximately 60{\%} of the CD8αβ T cells in the spleen and peripheral lymph nodes of naive DKO mice display a memory (CD44 high) phenotype. To investigate the origins of these non-class Ia-selected CD8αβCD44high cells, we traced the phenotype of recent thymic emigrants and found that most were CD44low. We also determined whether their appearance was thymus dependent and found that only a small percentage of non-class Ia-selected CD8αβCD44high cells develop in a thymus-independent pathway. Functionally, CD8αβCD44high cells from DKO mice are able to secrete IFN-γ in response to IL-12 and IL-18 in the absence of cognate Ag. When challenged with anti-CD3 in vivo, nearly half of these cells produce IFN-γ within 3 h. When panned CD8αβCD44high cells from Thy1.2.DKO mice were transferred into Thy1.1 DKO recipients and then challenged with Listeria monocytogenes, an Ag-specific anti-L. monocytogenes response was observed 6 days later. Our data suggest that non-class Ia-selected CD8αβCD44high cells in naive animals can respond rapidly to Ag and play a role in the innate as well as the early phase of the acquired immune response.",
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