Thyroid c cells in the digeorge anomaly

A quantitative study

Siegfried Pueblitz, Arthur G. Weinberg, Jorge Albores-Saavedra

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The developmental field defect in the DiGeorge anomaly (DGA) principally affects derivatives of the third and fourth branchial pouches (areas containing a large population of cephalic neural crest cells), including the ultimobranchial body (UB), the reputed source of thyroid calcitonin-producing cells (C cells) in humans. To evaluate the content of C cells in children with DGA, sections of the thyroid in 16 cases of DGA (group I) (8 incomplete and 8 complete forms) and 16 age-matched controls (group II) were stained by immunoperoxidase for calcitonin and chromogranin. Eleven of 16 (69% cases in group I [4 of 8 (50% of the complete and 7 of 8 (88% of the incomplete cases] and 14 of 16 (88% of group II exhibited positive-staining cells for both markers, either individually or in small clusters within the follicular epithelial basement membrane. The average number of C cells per high-power field (HPF) (400 x) for group I was 1.6 ± 0.9 and for group II 4.9 ± 1.7 (P < 005). Although the percentage of positive incomplete DGA cases was the same as that of the control cases, the average number of C cells/HPF was 2.0 ± 1.1 and similar to that of complete DGA cases (1.2 ± 0.6). These results demonstrated that C cells are present in the thyroid of patients with DGA more frequently than expected, although in deficient numbers when compared quantitatively to age-matched controls showing a normal infantile pattern of thyroid C cell distribution. Although this observation confirms that there is deficiency of thyroid C cell development in DGA and is in keeping with the assumption that the cells are of neural crest origin, our data raise the possibility of an additional source of C cells, perhaps from thyroid endoderm, in a manner analogous to the endocrine cells of the gut and respiratory tract..

Original languageEnglish (US)
Pages (from-to)463-473
Number of pages11
JournalFetal and Pediatric Pathology
Volume13
Issue number4
DOIs
StatePublished - 1993

Fingerprint

DiGeorge Syndrome
Calcitonin
Thyroid Gland
Neural Crest
Ultimobranchial Body
Enteroendocrine Cells
Chromogranins
Endoderm
Basement Membrane
Respiratory System
Research Design
Head
Staining and Labeling
Control Groups

Keywords

  • C-cells
  • Developmental field defect
  • DiGeorge anomaly
  • Thyroid

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Pediatrics, Perinatology, and Child Health

Cite this

Thyroid c cells in the digeorge anomaly : A quantitative study. / Pueblitz, Siegfried; Weinberg, Arthur G.; Albores-Saavedra, Jorge.

In: Fetal and Pediatric Pathology, Vol. 13, No. 4, 1993, p. 463-473.

Research output: Contribution to journalArticle

Pueblitz, S, Weinberg, AG & Albores-Saavedra, J 1993, 'Thyroid c cells in the digeorge anomaly: A quantitative study', Fetal and Pediatric Pathology, vol. 13, no. 4, pp. 463-473. https://doi.org/10.3109/15513819309048236
Pueblitz, Siegfried ; Weinberg, Arthur G. ; Albores-Saavedra, Jorge. / Thyroid c cells in the digeorge anomaly : A quantitative study. In: Fetal and Pediatric Pathology. 1993 ; Vol. 13, No. 4. pp. 463-473.
@article{bcc0abd192e249ba8633b38075601348,
title = "Thyroid c cells in the digeorge anomaly: A quantitative study",
abstract = "The developmental field defect in the DiGeorge anomaly (DGA) principally affects derivatives of the third and fourth branchial pouches (areas containing a large population of cephalic neural crest cells), including the ultimobranchial body (UB), the reputed source of thyroid calcitonin-producing cells (C cells) in humans. To evaluate the content of C cells in children with DGA, sections of the thyroid in 16 cases of DGA (group I) (8 incomplete and 8 complete forms) and 16 age-matched controls (group II) were stained by immunoperoxidase for calcitonin and chromogranin. Eleven of 16 (69{\%} cases in group I [4 of 8 (50{\%} of the complete and 7 of 8 (88{\%} of the incomplete cases] and 14 of 16 (88{\%} of group II exhibited positive-staining cells for both markers, either individually or in small clusters within the follicular epithelial basement membrane. The average number of C cells per high-power field (HPF) (400 x) for group I was 1.6 ± 0.9 and for group II 4.9 ± 1.7 (P < 005). Although the percentage of positive incomplete DGA cases was the same as that of the control cases, the average number of C cells/HPF was 2.0 ± 1.1 and similar to that of complete DGA cases (1.2 ± 0.6). These results demonstrated that C cells are present in the thyroid of patients with DGA more frequently than expected, although in deficient numbers when compared quantitatively to age-matched controls showing a normal infantile pattern of thyroid C cell distribution. Although this observation confirms that there is deficiency of thyroid C cell development in DGA and is in keeping with the assumption that the cells are of neural crest origin, our data raise the possibility of an additional source of C cells, perhaps from thyroid endoderm, in a manner analogous to the endocrine cells of the gut and respiratory tract..",
keywords = "C-cells, Developmental field defect, DiGeorge anomaly, Thyroid",
author = "Siegfried Pueblitz and Weinberg, {Arthur G.} and Jorge Albores-Saavedra",
year = "1993",
doi = "10.3109/15513819309048236",
language = "English (US)",
volume = "13",
pages = "463--473",
journal = "Fetal and Pediatric Pathology",
issn = "1551-3815",
publisher = "Informa Healthcare",
number = "4",

}

TY - JOUR

T1 - Thyroid c cells in the digeorge anomaly

T2 - A quantitative study

AU - Pueblitz, Siegfried

AU - Weinberg, Arthur G.

AU - Albores-Saavedra, Jorge

PY - 1993

Y1 - 1993

N2 - The developmental field defect in the DiGeorge anomaly (DGA) principally affects derivatives of the third and fourth branchial pouches (areas containing a large population of cephalic neural crest cells), including the ultimobranchial body (UB), the reputed source of thyroid calcitonin-producing cells (C cells) in humans. To evaluate the content of C cells in children with DGA, sections of the thyroid in 16 cases of DGA (group I) (8 incomplete and 8 complete forms) and 16 age-matched controls (group II) were stained by immunoperoxidase for calcitonin and chromogranin. Eleven of 16 (69% cases in group I [4 of 8 (50% of the complete and 7 of 8 (88% of the incomplete cases] and 14 of 16 (88% of group II exhibited positive-staining cells for both markers, either individually or in small clusters within the follicular epithelial basement membrane. The average number of C cells per high-power field (HPF) (400 x) for group I was 1.6 ± 0.9 and for group II 4.9 ± 1.7 (P < 005). Although the percentage of positive incomplete DGA cases was the same as that of the control cases, the average number of C cells/HPF was 2.0 ± 1.1 and similar to that of complete DGA cases (1.2 ± 0.6). These results demonstrated that C cells are present in the thyroid of patients with DGA more frequently than expected, although in deficient numbers when compared quantitatively to age-matched controls showing a normal infantile pattern of thyroid C cell distribution. Although this observation confirms that there is deficiency of thyroid C cell development in DGA and is in keeping with the assumption that the cells are of neural crest origin, our data raise the possibility of an additional source of C cells, perhaps from thyroid endoderm, in a manner analogous to the endocrine cells of the gut and respiratory tract..

AB - The developmental field defect in the DiGeorge anomaly (DGA) principally affects derivatives of the third and fourth branchial pouches (areas containing a large population of cephalic neural crest cells), including the ultimobranchial body (UB), the reputed source of thyroid calcitonin-producing cells (C cells) in humans. To evaluate the content of C cells in children with DGA, sections of the thyroid in 16 cases of DGA (group I) (8 incomplete and 8 complete forms) and 16 age-matched controls (group II) were stained by immunoperoxidase for calcitonin and chromogranin. Eleven of 16 (69% cases in group I [4 of 8 (50% of the complete and 7 of 8 (88% of the incomplete cases] and 14 of 16 (88% of group II exhibited positive-staining cells for both markers, either individually or in small clusters within the follicular epithelial basement membrane. The average number of C cells per high-power field (HPF) (400 x) for group I was 1.6 ± 0.9 and for group II 4.9 ± 1.7 (P < 005). Although the percentage of positive incomplete DGA cases was the same as that of the control cases, the average number of C cells/HPF was 2.0 ± 1.1 and similar to that of complete DGA cases (1.2 ± 0.6). These results demonstrated that C cells are present in the thyroid of patients with DGA more frequently than expected, although in deficient numbers when compared quantitatively to age-matched controls showing a normal infantile pattern of thyroid C cell distribution. Although this observation confirms that there is deficiency of thyroid C cell development in DGA and is in keeping with the assumption that the cells are of neural crest origin, our data raise the possibility of an additional source of C cells, perhaps from thyroid endoderm, in a manner analogous to the endocrine cells of the gut and respiratory tract..

KW - C-cells

KW - Developmental field defect

KW - DiGeorge anomaly

KW - Thyroid

UR - http://www.scopus.com/inward/record.url?scp=0027292007&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027292007&partnerID=8YFLogxK

U2 - 10.3109/15513819309048236

DO - 10.3109/15513819309048236

M3 - Article

VL - 13

SP - 463

EP - 473

JO - Fetal and Pediatric Pathology

JF - Fetal and Pediatric Pathology

SN - 1551-3815

IS - 4

ER -