Thyroid hormone stimulates the renal Na/H exchanger NHE3 by transcriptional activation

Adriana Cano, Michel Baum, Orson W. Moe

Research output: Contribution to journalArticle

Abstract

Thyroid hormone stimulates renal proximal tubule NaCl and NaHCO3 absorption in part by activating the apical membrane Na/H exchanger NHE3. We used a renal epithelial cell line, the opossum kidney (OK) cell, to define the mechanism by which 3,5,3′-triiodothyronine (T3) increases NHE3 activity. T3 stimulated NHE3 activity, an effect that was blocked by inhibition of cellular transcription or translation. The increase in activity was associated with increases in steady-state cell surface and total cellular NHE3 protein and NHE3 transcript abundance. T3 stimulated transcription of the NHE3 gene and had no effect on NHE3 transcript stability. The transcriptional activity of the 5′-flanking region of the rat NHE3 gene was stimulated by T3 when expressed in OK cells. When heterologously expressed rat NHE3 transcript levels were clamped constant with a constitutive promoter in OK cells, T3 has no effect on rat NHE3 protein abundance, suggesting the absence of regulation of NHE3 protein stability or translation. These studies demonstrate that T3 stimulates NHE3 activity by activating NHE3 gene transcription and increasing NHE3 transcript and protein abundance.

Original languageEnglish (US)
JournalAmerican Journal of Physiology
Volume276
Issue number1 PART 1
StatePublished - Jan 1999

Fingerprint

Sodium-Hydrogen Antiporter
Thyroid Hormones
Opossums
Transcriptional Activation
Kidney
Genes
Proximal Kidney Tubule
Proteins
Protein Stability
5' Flanking Region
Protein Biosynthesis
Triiodothyronine
Epithelial Cells
Cell Line
Membranes

Keywords

  • Acid-base balance
  • Development
  • NaCl homeostasis
  • Promoter
  • Proximal tubule

ASJC Scopus subject areas

  • Physiology (medical)

Cite this

Thyroid hormone stimulates the renal Na/H exchanger NHE3 by transcriptional activation. / Cano, Adriana; Baum, Michel; Moe, Orson W.

In: American Journal of Physiology, Vol. 276, No. 1 PART 1, 01.1999.

Research output: Contribution to journalArticle

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