Tight junctions and enteropathogenic E. coli

Andrew W. Weflen, Neal M. Alto, Gail A. Hecht

Research output: Chapter in Book/Report/Conference proceedingChapter

19 Scopus citations

Abstract

Enteropathogenic E. coli (EPEC) are a leading cause of infantile diarrhea in developing countries, resulting in millions of deaths each year. EPEC secrete virulence factors, also called effectors, directly into host intestinal epithelial cells via type three secretion systems. Secreted effectors then affect host signaling pathways to induce several phenotypes, which ultimately lead to disease. Among the over 20 secreted effectors is E. coli secreted protein F (EspF), a 206 amino acid protein believed to be central to EPEC pathogenesis, as it disrupts tight junction structure and function. Although the mechanism by which this occurs is unknown, EspF has recently been found to contain several protein-protein interaction domains that may be involved. We have shown EspF to interact with the endocytic regulators sorting nexin 9 (SNX9) and N-WASP via non-exclusive binding sites. These interactions induce actin polymerization in vitro, and interaction with SNX9 alters its endocytic activity, as EspF induces the formation of tubular vesicles in a manner dependent upon its interaction with SNX9. EspF, therefore, appears to hijack endocytic regulation via SNX9 and possibly N-WASP interaction, to affect an as yet unidentified pathogenic phenotype.

Original languageEnglish (US)
Title of host publicationMolecular Structure and Function of the Tight Junction From Basic Mechanisms to Clinical Manifestations
PublisherBlackwell Publishing Inc.
Pages169-174
Number of pages6
ISBN (Print)9781573317498
DOIs
StatePublished - May 2009

Publication series

NameAnnals of the New York Academy of Sciences
Volume1165
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Keywords

  • EPEC
  • EspF
  • SNX9
  • Tight junction

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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    Weflen, A. W., Alto, N. M., & Hecht, G. A. (2009). Tight junctions and enteropathogenic E. coli. In Molecular Structure and Function of the Tight Junction From Basic Mechanisms to Clinical Manifestations (pp. 169-174). (Annals of the New York Academy of Sciences; Vol. 1165). Blackwell Publishing Inc.. https://doi.org/10.1111/j.1749-6632.2009.04060.x