Tight junctions and enteropathogenic E. coli

Andrew W. Weflen, Neal M. Alto, Gail A. Hecht

Research output: Chapter in Book/Report/Conference proceedingChapter

19 Citations (Scopus)

Abstract

Enteropathogenic E. coli (EPEC) are a leading cause of infantile diarrhea in developing countries, resulting in millions of deaths each year. EPEC secrete virulence factors, also called effectors, directly into host intestinal epithelial cells via type three secretion systems. Secreted effectors then affect host signaling pathways to induce several phenotypes, which ultimately lead to disease. Among the over 20 secreted effectors is E. coli secreted protein F (EspF), a 206 amino acid protein believed to be central to EPEC pathogenesis, as it disrupts tight junction structure and function. Although the mechanism by which this occurs is unknown, EspF has recently been found to contain several protein-protein interaction domains that may be involved. We have shown EspF to interact with the endocytic regulators sorting nexin 9 (SNX9) and N-WASP via non-exclusive binding sites. These interactions induce actin polymerization in vitro, and interaction with SNX9 alters its endocytic activity, as EspF induces the formation of tubular vesicles in a manner dependent upon its interaction with SNX9. EspF, therefore, appears to hijack endocytic regulation via SNX9 and possibly N-WASP interaction, to affect an as yet unidentified pathogenic phenotype.

Original languageEnglish (US)
Title of host publicationAnnals of the New York Academy of Sciences
Pages169-174
Number of pages6
Volume1165
DOIs
StatePublished - May 2009

Publication series

NameAnnals of the New York Academy of Sciences
Volume1165
ISSN (Print)00778923
ISSN (Electronic)17496632

Fingerprint

Sorting Nexins
Enteropathogenic Escherichia coli
Tight Junctions
Escherichia coli Proteins
Escherichia coli
Proteins
Infantile Diarrhea
Protein Interaction Domains and Motifs
Phenotype
Virulence Factors
Polymerization
Developing Countries
Actins
Epithelial Cells
Binding Sites
Amino Acids
Developing countries

Keywords

  • EPEC
  • EspF
  • SNX9
  • Tight junction

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Weflen, A. W., Alto, N. M., & Hecht, G. A. (2009). Tight junctions and enteropathogenic E. coli. In Annals of the New York Academy of Sciences (Vol. 1165, pp. 169-174). (Annals of the New York Academy of Sciences; Vol. 1165). https://doi.org/10.1111/j.1749-6632.2009.04060.x

Tight junctions and enteropathogenic E. coli. / Weflen, Andrew W.; Alto, Neal M.; Hecht, Gail A.

Annals of the New York Academy of Sciences. Vol. 1165 2009. p. 169-174 (Annals of the New York Academy of Sciences; Vol. 1165).

Research output: Chapter in Book/Report/Conference proceedingChapter

Weflen, AW, Alto, NM & Hecht, GA 2009, Tight junctions and enteropathogenic E. coli. in Annals of the New York Academy of Sciences. vol. 1165, Annals of the New York Academy of Sciences, vol. 1165, pp. 169-174. https://doi.org/10.1111/j.1749-6632.2009.04060.x
Weflen AW, Alto NM, Hecht GA. Tight junctions and enteropathogenic E. coli. In Annals of the New York Academy of Sciences. Vol. 1165. 2009. p. 169-174. (Annals of the New York Academy of Sciences). https://doi.org/10.1111/j.1749-6632.2009.04060.x
Weflen, Andrew W. ; Alto, Neal M. ; Hecht, Gail A. / Tight junctions and enteropathogenic E. coli. Annals of the New York Academy of Sciences. Vol. 1165 2009. pp. 169-174 (Annals of the New York Academy of Sciences).
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