Tightly regulated gene expression system in Salmonella enterica serovar Typhimurium

Jeffrey McKinney, Cecilia Guerrier-Takada, Jorge Galán, Sidney Altman

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A new Salmonella enterica serovar Typhimurium strain has been constructed to facilitate tightly regulated gene expression. Arabinose-inducible and glucose-repressible expression of a T7 RNA polymerase gene that has been integrated with an adjacent araC-PBAD control element into the bacterial chromosome allows dynamic control of T7 promoter-driven RNA transcription.

Original languageEnglish (US)
Pages (from-to)6056-6059
Number of pages4
JournalJournal of Bacteriology
Volume184
Issue number21
DOIs
StatePublished - Nov 2002

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Bacterial Chromosomes
Arabinose
Salmonella enterica
RNA
Gene Expression
Glucose
Genes
Serogroup
bacteriophage T7 RNA polymerase

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Tightly regulated gene expression system in Salmonella enterica serovar Typhimurium. / McKinney, Jeffrey; Guerrier-Takada, Cecilia; Galán, Jorge; Altman, Sidney.

In: Journal of Bacteriology, Vol. 184, No. 21, 11.2002, p. 6056-6059.

Research output: Contribution to journalArticle

McKinney, Jeffrey ; Guerrier-Takada, Cecilia ; Galán, Jorge ; Altman, Sidney. / Tightly regulated gene expression system in Salmonella enterica serovar Typhimurium. In: Journal of Bacteriology. 2002 ; Vol. 184, No. 21. pp. 6056-6059.
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