TY - JOUR
T1 - Time-course magnetic resonance imaging of rat pancreatic cyst after experimental pancreatitis
AU - Seki, Yasunaga
AU - Naruse, Satoru
AU - Seo, Yoshiteru
AU - Kitagawa, Motoji
AU - Ishiguro, Hiroshi
AU - Wang, Youxue
AU - Murakami, Masataka
AU - Hayakawa, Tetsuo
N1 - Funding Information:
This work was supported by a grant from the Ministry of Education, Science, and Culture, Japan and a Joint Research Project for magnetic resonance imaging and spectroscopy room in National Institute for Physiological Sciences. The authors thank Dr. T. Ozaki, N. Ogiso, T. Sazi, and T. Hiroe for their support on animal care, Mr. H. Okawara, M. Takagi, for their technical assistance on MR imaging and Dr. M. Nagahama for his advice on histology.
PY - 2000
Y1 - 2000
N2 - Fast magnetic resonance (MR) imaging of the rat pancreas was carried out using a snapshot method to observe three-dimensional (3D) and temporal development of the pancreatic cyst after experimental pancreatitis. Acute pancreatitis was induced by a retrograde infusion of the trypsin-taurocholate solution into the pancreatic duct in 23 rats, of which seven survived for one month. Under 2% enflurane anesthesia, 1H images of the rat abdomen were taken by a 4.7 T magnetic resonance spectrometer under spontaneous breathing. 3D images of the pancreas and cyst were reconstructed from the axial, sagittal and coronal images taken before, 24 h, 7 days, 14 days, 21 days and 28 days after the induction of pancreatitis. The 3D images reconstructed from different slice orientations at each time point showed good agreement with each other. The calculated volumes of the cyst on 7th, 14th, 21st, and 28th day were 0.3 ± 0.1, 0.8 ± 0.3, 2.1 ± 0.6, 6.5 ± 1.3 mL, respectively. The cystic fluid volume on 28th day was 6.4 ± 1.4 mL, which confirmed reliability of volume measurement by MR imaging. Fast MR imaging (snapshot) together with 3D reconstruction allows us to understand the detailed chronological and spatial development of pancreatic cyst after acute pancreatitis in rats. (C) 2000 Elsevier Science Inc.
AB - Fast magnetic resonance (MR) imaging of the rat pancreas was carried out using a snapshot method to observe three-dimensional (3D) and temporal development of the pancreatic cyst after experimental pancreatitis. Acute pancreatitis was induced by a retrograde infusion of the trypsin-taurocholate solution into the pancreatic duct in 23 rats, of which seven survived for one month. Under 2% enflurane anesthesia, 1H images of the rat abdomen were taken by a 4.7 T magnetic resonance spectrometer under spontaneous breathing. 3D images of the pancreas and cyst were reconstructed from the axial, sagittal and coronal images taken before, 24 h, 7 days, 14 days, 21 days and 28 days after the induction of pancreatitis. The 3D images reconstructed from different slice orientations at each time point showed good agreement with each other. The calculated volumes of the cyst on 7th, 14th, 21st, and 28th day were 0.3 ± 0.1, 0.8 ± 0.3, 2.1 ± 0.6, 6.5 ± 1.3 mL, respectively. The cystic fluid volume on 28th day was 6.4 ± 1.4 mL, which confirmed reliability of volume measurement by MR imaging. Fast MR imaging (snapshot) together with 3D reconstruction allows us to understand the detailed chronological and spatial development of pancreatic cyst after acute pancreatitis in rats. (C) 2000 Elsevier Science Inc.
KW - 3D reconstruction
KW - Experimental pancreatitis
KW - Pancreatic cyst
KW - Rat
KW - Snapshot
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U2 - 10.1016/S0730-725X(00)00203-4
DO - 10.1016/S0730-725X(00)00203-4
M3 - Article
C2 - 11121705
AN - SCOPUS:0033659292
SN - 0730-725X
VL - 18
SP - 1003
EP - 1010
JO - Magnetic Resonance Imaging
JF - Magnetic Resonance Imaging
IS - 8
ER -