Time-dependent changes in spontaneous respiratory activity in turtle brainstems in vitro

J. E R Wilkerson, M. R. Wenninger, G. S. Mitchell, S. M. Johnson

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Our goal was to determine whether time-dependent changes in respiratory motor output in vitro could be minimized by altering bath solution composition. Adult turtle brainstems were bathed in standard solution, nutrient-rich Dulbecco's Eagle media (100 or 25% concentration), or standard solution with phenylbiguanide (PBG, 5-HT3 agonist which increases respiratory drive). Except for a 63% frequency increase in PBG solution, hypoglossal bursts were unaltered within 100 min of observation. Respiratory activity was abolished within 7 h in 100% Dulbecco's compared with a mean of 24-31 h in other test solutions. At 12 h, burst frequency decreased faster in standard solution and 25% Dulbecco's (-0.28±0.07 and -0.13±0.05 bursts/h, respectively) compared with PBG solution (-0.09±0.04 bursts/h); amplitude declined at ∼2%/h in all solutions. The tendency for episodic discharge decreased gradually in standard solution, but was eliminated in 25% Dulbecco's and PBG solution. Certain bath solutions may minimize time-dependent frequency reductions but may also cause breathing pattern changes.

Original languageEnglish (US)
Pages (from-to)253-263
Number of pages11
JournalRespiratory Physiology and Neurobiology
Volume138
Issue number2-3
DOIs
StatePublished - Nov 14 2003

Fingerprint

Turtles
Brain Stem
Baths
In Vitro Techniques
Serotonin 5-HT3 Receptor Agonists
Eagles
Respiration
Observation

Keywords

  • Control of breathing, respiratory motor output
  • Methods, respiratory motor output, bath solution
  • Reptiles, red-eared slider turtle (Trachemys scripta)

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Neuroscience(all)

Cite this

Time-dependent changes in spontaneous respiratory activity in turtle brainstems in vitro. / Wilkerson, J. E R; Wenninger, M. R.; Mitchell, G. S.; Johnson, S. M.

In: Respiratory Physiology and Neurobiology, Vol. 138, No. 2-3, 14.11.2003, p. 253-263.

Research output: Contribution to journalArticle

Wilkerson, J. E R ; Wenninger, M. R. ; Mitchell, G. S. ; Johnson, S. M. / Time-dependent changes in spontaneous respiratory activity in turtle brainstems in vitro. In: Respiratory Physiology and Neurobiology. 2003 ; Vol. 138, No. 2-3. pp. 253-263.
@article{cdbba2d901dd4073af3cacd957b57a02,
title = "Time-dependent changes in spontaneous respiratory activity in turtle brainstems in vitro",
abstract = "Our goal was to determine whether time-dependent changes in respiratory motor output in vitro could be minimized by altering bath solution composition. Adult turtle brainstems were bathed in standard solution, nutrient-rich Dulbecco's Eagle media (100 or 25{\%} concentration), or standard solution with phenylbiguanide (PBG, 5-HT3 agonist which increases respiratory drive). Except for a 63{\%} frequency increase in PBG solution, hypoglossal bursts were unaltered within 100 min of observation. Respiratory activity was abolished within 7 h in 100{\%} Dulbecco's compared with a mean of 24-31 h in other test solutions. At 12 h, burst frequency decreased faster in standard solution and 25{\%} Dulbecco's (-0.28±0.07 and -0.13±0.05 bursts/h, respectively) compared with PBG solution (-0.09±0.04 bursts/h); amplitude declined at ∼2{\%}/h in all solutions. The tendency for episodic discharge decreased gradually in standard solution, but was eliminated in 25{\%} Dulbecco's and PBG solution. Certain bath solutions may minimize time-dependent frequency reductions but may also cause breathing pattern changes.",
keywords = "Control of breathing, respiratory motor output, Methods, respiratory motor output, bath solution, Reptiles, red-eared slider turtle (Trachemys scripta)",
author = "Wilkerson, {J. E R} and Wenninger, {M. R.} and Mitchell, {G. S.} and Johnson, {S. M.}",
year = "2003",
month = "11",
day = "14",
doi = "10.1016/j.resp.2003.08.006",
language = "English (US)",
volume = "138",
pages = "253--263",
journal = "Respiratory Physiology and Neurobiology",
issn = "1569-9048",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - Time-dependent changes in spontaneous respiratory activity in turtle brainstems in vitro

AU - Wilkerson, J. E R

AU - Wenninger, M. R.

AU - Mitchell, G. S.

AU - Johnson, S. M.

PY - 2003/11/14

Y1 - 2003/11/14

N2 - Our goal was to determine whether time-dependent changes in respiratory motor output in vitro could be minimized by altering bath solution composition. Adult turtle brainstems were bathed in standard solution, nutrient-rich Dulbecco's Eagle media (100 or 25% concentration), or standard solution with phenylbiguanide (PBG, 5-HT3 agonist which increases respiratory drive). Except for a 63% frequency increase in PBG solution, hypoglossal bursts were unaltered within 100 min of observation. Respiratory activity was abolished within 7 h in 100% Dulbecco's compared with a mean of 24-31 h in other test solutions. At 12 h, burst frequency decreased faster in standard solution and 25% Dulbecco's (-0.28±0.07 and -0.13±0.05 bursts/h, respectively) compared with PBG solution (-0.09±0.04 bursts/h); amplitude declined at ∼2%/h in all solutions. The tendency for episodic discharge decreased gradually in standard solution, but was eliminated in 25% Dulbecco's and PBG solution. Certain bath solutions may minimize time-dependent frequency reductions but may also cause breathing pattern changes.

AB - Our goal was to determine whether time-dependent changes in respiratory motor output in vitro could be minimized by altering bath solution composition. Adult turtle brainstems were bathed in standard solution, nutrient-rich Dulbecco's Eagle media (100 or 25% concentration), or standard solution with phenylbiguanide (PBG, 5-HT3 agonist which increases respiratory drive). Except for a 63% frequency increase in PBG solution, hypoglossal bursts were unaltered within 100 min of observation. Respiratory activity was abolished within 7 h in 100% Dulbecco's compared with a mean of 24-31 h in other test solutions. At 12 h, burst frequency decreased faster in standard solution and 25% Dulbecco's (-0.28±0.07 and -0.13±0.05 bursts/h, respectively) compared with PBG solution (-0.09±0.04 bursts/h); amplitude declined at ∼2%/h in all solutions. The tendency for episodic discharge decreased gradually in standard solution, but was eliminated in 25% Dulbecco's and PBG solution. Certain bath solutions may minimize time-dependent frequency reductions but may also cause breathing pattern changes.

KW - Control of breathing, respiratory motor output

KW - Methods, respiratory motor output, bath solution

KW - Reptiles, red-eared slider turtle (Trachemys scripta)

UR - http://www.scopus.com/inward/record.url?scp=0242438684&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0242438684&partnerID=8YFLogxK

U2 - 10.1016/j.resp.2003.08.006

DO - 10.1016/j.resp.2003.08.006

M3 - Article

VL - 138

SP - 253

EP - 263

JO - Respiratory Physiology and Neurobiology

JF - Respiratory Physiology and Neurobiology

SN - 1569-9048

IS - 2-3

ER -