Time-dependent whole-body fluorescence tomography of probe bio-distributions in mice

Sachin V. Patwardhan, Sharon R. Bloch, Samuel Achilefu, Joseph P. Culver

Research output: Contribution to journalArticlepeer-review

176 Scopus citations

Abstract

We present a fast scanning fluorescence optical tomography system for imaging the kinetics of probe distributions through out the whole body of small animals. Configured in a plane parallel geometry, the system scans a source laser using a galvanometer mirror pair (τsWitch∼1ms) over flexible source patterns, and detects excitation and emission light using a high frame rate low noise, 5 MHz electron multiplied charge-coupled device (EMCCD) camera. Phantom studies were used to evaluate resolution, linearity, and sensitivity. Time dependent (δτ=2.2 min.) in vivo imaging of mice was performed following injections of a fluorescing probe (indocyanine green). The capability to detect differences in probe delivery route was demonstrated by comparing an intravenous injection, versus an injection into a fat pocket (retro orbital injection). Feasibility of imaging the distribution of tumor-targeted molecular probes was demonstrated by imaging a breast tumor-specific near infrared polypeptide in MDA MB 361 tumor bearing nude mice. A tomography scan, at 24 hour post injection, revealed preferential uptake in the tumor relative to surrounding tissue.

Original languageEnglish (US)
Pages (from-to)2564-2571
Number of pages8
JournalOptics Express
Volume13
Issue number7
DOIs
StatePublished - Apr 2005
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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