Time-Lapse Monitoring of DNA Damage Colocalized With Particle Tracks in Single Living Cells

Conor H. McFadden, Timothy M. Hallacy, David B. Flint, Dal A. Granville, Aroumougame Asaithamby, Narayan Sahoo, Mark S. Akselrod, Gabriel O. Sawakuchi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Purpose Understanding the DNA damage and repair induced by hadron therapy (HT) beams is crucial for developing novel strategies to maximize the use of HT beams to treat cancer patients. However, spatiotemporal studies of DNA damage and repair for beam energies relevant to HT have been challenging. We report a technique that enables spatiotemporal measurement of radiation-induced damage in live cells and colocalization of this damage with charged particle tracks over a broad range of clinically relevant beam energies. The technique uses novel fluorescence nuclear track detectors with fluorescence confocal laser scanning microscopy in the beam line to visualize particle track traversals within the subcellular compartments of live cells within seconds after injury. Methods and Materials We designed and built a portable fluorescence confocal laser scanning microscope for use in the beam path, coated fluorescence nuclear track detectors with fluorescent-tagged live cells (HT1080 expressing enhanced green fluorescent protein tagged to XRCC1, a single-strand break repair protein), placed the entire assembly into a proton therapy beam line, and irradiated the cells with a fluence of ∼1 × 106 protons/cm2. Results We successfully obtained confocal images of proton tracks and foci of DNA single-strand breaks immediately after irradiation. Conclusions This technique represents an innovative method for analyzing biological responses in any HT beam line at energies and dose rates relevant to therapy. It allows precise determination of the number of tracks traversing a subcellular compartment and monitoring the cellular damage therein, and has the potential to measure the linear energy transfer of each track from therapeutic beams.

Original languageEnglish (US)
Pages (from-to)221-227
Number of pages7
JournalInternational Journal of Radiation Oncology Biology Physics
Volume96
Issue number1
DOIs
StatePublished - Sep 1 2016

Fingerprint

particle tracks
DNA Damage
deoxyribonucleic acid
damage
therapy
Fluorescence
DNA Repair
Protons
fluorescence
Therapeutics
compartments
Proton Therapy
cells
Linear Energy Transfer
Single-Stranded DNA Breaks
strands
protons
Confocal Microscopy
proteins
linear energy transfer (LET)

ASJC Scopus subject areas

  • Radiation
  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

Cite this

Time-Lapse Monitoring of DNA Damage Colocalized With Particle Tracks in Single Living Cells. / McFadden, Conor H.; Hallacy, Timothy M.; Flint, David B.; Granville, Dal A.; Asaithamby, Aroumougame; Sahoo, Narayan; Akselrod, Mark S.; Sawakuchi, Gabriel O.

In: International Journal of Radiation Oncology Biology Physics, Vol. 96, No. 1, 01.09.2016, p. 221-227.

Research output: Contribution to journalArticle

McFadden, Conor H. ; Hallacy, Timothy M. ; Flint, David B. ; Granville, Dal A. ; Asaithamby, Aroumougame ; Sahoo, Narayan ; Akselrod, Mark S. ; Sawakuchi, Gabriel O. / Time-Lapse Monitoring of DNA Damage Colocalized With Particle Tracks in Single Living Cells. In: International Journal of Radiation Oncology Biology Physics. 2016 ; Vol. 96, No. 1. pp. 221-227.
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