Tissue distribution and localization of natriuretic peptide receptor subtypes in stroke-prone spontaneously hypertensive rats

Miki Nagase, Takeshi Katafuchi, Shigehisa Hirose, Toshiro Fujita

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Objective. To investigate tissue distribution and localization of the natriuretic peptide receptor (NPR) subtypes' messenger RNA (mRNA) and to compare their expression between stroke-prone spontaneously hypertensive rats (SHR-SP) and Wistar-Kyoto (WKY) rats. Methods. Total RNA was extracted from organs of SHR-SP and WKY rats aged 13 weeks. The mRNA level was examined by RNase protection assay. The localization of the transcripts was determined by in-situ hybridization. Results. In SHR-SP aged 13 weeks. NPR-A was expressed most abundantly in the adrenal gland, lung and aorta, in that order, NPR-B was expressed highly in the uterus and ovary, and also in the lung, adrenal, and brain. NPR-C was expressed predominantly in the atrium and mesentery, less so in the lung, vein, and kidney. In the adrenal gland, NPR-A was expressed mainly in zona glomerulosa cells. In the atrium, NPR-C was expressed throughout the wall. In the mesentery, NPR-C mRNA was detected mainly in adipocytes. In the kidney, NPR-C was found predominantly in podocytes. Whereas the levels of expression of NPR subtypes in most tissues examined did not differ between SHR-SP and WKY rats, the NPR-C mRNA level was significantly greater in the kidneys of SHR-SP than it was in those of WKY rats. Conclusions. These results indicated that each NPR subtype had a distinct tissue distribution pattern and that the expression of NPR-C in the kidneys of SHR-SP was greater than that in the kidneys of WKY rats.

Original languageEnglish (US)
Pages (from-to)1235-1243
Number of pages9
JournalJournal of hypertension
Volume15
Issue number11
DOIs
StatePublished - 1997

Keywords

  • Atrial natriuretic factor
  • In-situ hybridization
  • RNase protection assay
  • Receptors
  • Stroke-prone spontaneously hypertensive rats
  • Wistar-Kyoto rats

ASJC Scopus subject areas

  • Internal Medicine
  • Physiology
  • Cardiology and Cardiovascular Medicine

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