Tissue distribution and localization of natriuretic peptide receptor subtypes in stroke-prone spontaneously hypertensive rats

Miki Nagase, Takeshi Katafuchi, Shigehisa Hirose, Toshiro Fujita

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Objective. To investigate tissue distribution and localization of the natriuretic peptide receptor (NPR) subtypes' messenger RNA (mRNA) and to compare their expression between stroke-prone spontaneously hypertensive rats (SHR-SP) and Wistar-Kyoto (WKY) rats. Methods. Total RNA was extracted from organs of SHR-SP and WKY rats aged 13 weeks. The mRNA level was examined by RNase protection assay. The localization of the transcripts was determined by in-situ hybridization. Results. In SHR-SP aged 13 weeks. NPR-A was expressed most abundantly in the adrenal gland, lung and aorta, in that order, NPR-B was expressed highly in the uterus and ovary, and also in the lung, adrenal, and brain. NPR-C was expressed predominantly in the atrium and mesentery, less so in the lung, vein, and kidney. In the adrenal gland, NPR-A was expressed mainly in zona glomerulosa cells. In the atrium, NPR-C was expressed throughout the wall. In the mesentery, NPR-C mRNA was detected mainly in adipocytes. In the kidney, NPR-C was found predominantly in podocytes. Whereas the levels of expression of NPR subtypes in most tissues examined did not differ between SHR-SP and WKY rats, the NPR-C mRNA level was significantly greater in the kidneys of SHR-SP than it was in those of WKY rats. Conclusions. These results indicated that each NPR subtype had a distinct tissue distribution pattern and that the expression of NPR-C in the kidneys of SHR-SP was greater than that in the kidneys of WKY rats.

Original languageEnglish (US)
Pages (from-to)1235-1243
Number of pages9
JournalJournal of hypertension
Volume15
Issue number11
DOIs
StatePublished - 1997

Keywords

  • Atrial natriuretic factor
  • In-situ hybridization
  • RNase protection assay
  • Receptors
  • Stroke-prone spontaneously hypertensive rats
  • Wistar-Kyoto rats

ASJC Scopus subject areas

  • Internal Medicine
  • Physiology
  • Cardiology and Cardiovascular Medicine

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