Tissue distribution of breast cancer-associated antigens defined by monoclonal antibodies

A. E. Frankel, D. B. Ring, F. Tringale, S. T. Hsieh-Ma

Research output: Contribution to journalArticlepeer-review

95 Scopus citations

Abstract

Balb/c mice were immunized with membrane preparations of primary and metastatic breast carcinomas or with live breast cancer cell lines. Sixty-two fusions were performed between immune splenocytes and SP2/0 mouse myeloma cells, and 107 hybridomas were cloned that produced antibody reactive with breast cancer membrane extract, cell lines, and frozen sections, but not with normal tissue membrane extracts or a human fibroblast line. Ninety-four monoclonal antibodies were purified and tested for binding to 16 normal tissue frozen sections and five blood cell types. Thirty-five of the 94 antibodies were also tested on breast cancer sections from 21 patients, 14 breast cancer cell lines, and 11 non-breast tumor sections. Two of 94 antibodies showed no reactivity to any of the 21 normal tissues or 11 nonbreast neoplasms studied. These two antibodies, 451B7 and 452F2, bound 60% of the breast cancer cell lines and 25% of the breast cancer tissue sections. Eight additional antibodies bound three or fewer of 21 normal tissues. These antibodies bound 25-85% of breast cancer sections, 0-75% of breast cancer cell lines, and many of the nonbreast cancers. A comparison of normal tissues and nonbreast tumors bound by the breast cancer-reactive antibodies indicated that most of the cross-reacting normal tissue structures were epithelial in origin, and that the most cross-reactive nonbreast cancers were those of secondary sex organs (uterus, prostate, ovary).

Original languageEnglish (US)
Pages (from-to)273-286
Number of pages14
JournalJournal of Biological Response Modifiers
Volume4
Issue number3
StatePublished - Jun 1985

Keywords

  • Antigen
  • Breast carcinoma
  • Monoclonal antibody
  • Normal tissue

ASJC Scopus subject areas

  • Immunology
  • Pharmacology
  • Cancer Research

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