Tissue engineered intrasynovial tendons: In vivo graft survival and tensile strength

Andrew Y. Zhang, Johan Thorfinn, Sepideh Saber, Ioannis K. Angelidis, Sae H. Ki, Hung M. Pham, James Chang

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Rabbit flexor tendons can be harvested, acellularized, and used as scaffolds for reseeding of flexor tendon constructs. The purpose of this study was to compare the biomechanical characteristics of seeded and unseeded flexor tendon constructs to autologous grafts in vivo in the rabbit model. The experimental groups were created using acellularized rabbit tendon scaffolds: scaffolds with no cells, and scaffolds re-seeded with either tenocytes or adipoderived stem cells. Autologous flexor tendon grafts were used as controls. All constructs bridged a zone II defect in the rabbit and were explanted at 2, 4, 10, or 20 weeks. These tendons were then tested for ultimate tensile strength (UTS) or processed for histology. The experimental groups exhibited UTS comparable to autologous grafts up to 4 weeks, and were significantly weaker at 10 weeks (p < 0.000-0.024). At 20 weeks, UTS was significantly improved (p = 0.013) in tenocyte-seeded constructs compared with 10 weeks. There was no significant difference in UTS between seeded or unseeded constructs at 2, 4, and 20 weeks. In all groups, there were more cells at the repair ends, and the cells penetrated to the core of the grafts over time. The goal of this technology is the development of tissue engineered tendon grafts for clinical use. This study provides proof of the concept that tissue-engineered flexor tendons-both acellularized and reseeded-can survive and incorporate over time. Further work will focus on bioreactor preconditioning of reseeded constructs to increase ultimate tensile strength.

Original languageEnglish (US)
Pages (from-to)283-289
Number of pages7
JournalEuropean Journal of Plastic Surgery
Volume33
Issue number5
DOIs
Publication statusPublished - Oct 1 2010

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Keywords

  • Acellularization
  • Biomechanical testing
  • Cell culture
  • Hand flexor tendon
  • Scaffold
  • Stem cells
  • Tenocytes
  • Tissue engineering

ASJC Scopus subject areas

  • Surgery

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