Tissue inhibitor of metalloproteinase-3 plays important roles in the kidney following unilateral ureteral obstruction

Hidenobu Kawamoto, Osamu Yasuda, Takashi Suzuki, Tohru Ozaki, Takamori Yotsui, Masayoshi Higuchi, Hiromi Rakugi, Keisuke Fukuo, Toshio Ogihara, Nobuyo Maeda

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Tissue inhibitor of metalloproteinase-3 (Timp-3), an inhibitor of matrix-degrading enzymes, is an important molecule for maintenance of the extracellular matrix. In this study, we generated Timp-3-deficient mice and used them to examine the effect of Timp-3-deficiency on blood pressure and to investigate the role of Timp-3 in the kidney following unilateral ureteral obstruction. The blood pressure and heart rate of Timp-3-deficient mice were not significantly different from those of wild-type mice. On the other hand, the obstructed kidneys of Timp-3-deficient mice developed more severe hydronephrosis than those of wild-type animals. Matrix metalloproteinase activities assessed by in situ zymography and transforming growth factor-β expression were elevated in Timp-3-deficient mice. The renal tissues were thinner and the ratio of renal medulla to cortex was significantly lower in the obstructed Timp-3-deficient kidneys. These findings indicate that Timp-3-deficiency does not substantially affect the blood pressure in mice, and that Timp-3 plays an important role in the maintenance of renal macrostructure after unilateral ureteral obstruction.

Original languageEnglish (US)
Pages (from-to)285-294
Number of pages10
JournalHypertension Research
Volume29
Issue number4
DOIs
StatePublished - Apr 2006
Externally publishedYes

Keywords

  • Extracellular matrix
  • Hydronephrosis
  • Matrix metalloproteinase
  • Tissue inhibitor of metalloproteinase-3
  • Unilateral ureteral obstruction

ASJC Scopus subject areas

  • Internal Medicine
  • Physiology
  • Cardiology and Cardiovascular Medicine

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