Tissue-specific expression of the rat pancreatic elastase I gene in transgenic mice

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

The gene for rat pancreatic elastase I is selectively expressed to high levels in the rat exocrine pancreas. When the cloned rat elastase I gene with 7 kb upstream and 5 kb downstream flanking sequences was introduced into mice by microinjection into fertilized eggs, the gene was expressed in a pancreas-specific manner. In four of five transgenic mice, the level of rat elastase I mRNA in the pancreas was equal to or greater than the normal rat level (10,000 mRNAs per cell) and correlated with the number of integrated gene copies. In nonpancreatic tissues the levels were at least 103-fold lower, except for expression in the liver of one mouse. Thus transfer of a 23 kb genomic DNA segment containing the rat elastase I gene to a foreign chromosomal location in the mouse can give rise to qualitatively and quantitatively normal expression.

Original languageEnglish (US)
Pages (from-to)639-646
Number of pages8
JournalCell
Volume38
Issue number3
DOIs
StatePublished - 1984

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Pancreatic Elastase
Transgenic Mice
Rats
Genes
Tissue
Pancreas
Exocrine Pancreas
Messenger RNA
Gene Dosage
Zygote
Microinjections
Liver
DNA

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Tissue-specific expression of the rat pancreatic elastase I gene in transgenic mice. / Swift, Galvin H; Hammer, Robert E; MacDonald, Raymond J; Brinster, Ralph L.

In: Cell, Vol. 38, No. 3, 1984, p. 639-646.

Research output: Contribution to journalArticle

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abstract = "The gene for rat pancreatic elastase I is selectively expressed to high levels in the rat exocrine pancreas. When the cloned rat elastase I gene with 7 kb upstream and 5 kb downstream flanking sequences was introduced into mice by microinjection into fertilized eggs, the gene was expressed in a pancreas-specific manner. In four of five transgenic mice, the level of rat elastase I mRNA in the pancreas was equal to or greater than the normal rat level (10,000 mRNAs per cell) and correlated with the number of integrated gene copies. In nonpancreatic tissues the levels were at least 103-fold lower, except for expression in the liver of one mouse. Thus transfer of a 23 kb genomic DNA segment containing the rat elastase I gene to a foreign chromosomal location in the mouse can give rise to qualitatively and quantitatively normal expression.",
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