Tissue specificity, localization in brain, and cell-free translation of mRNA encoding the A3 isoform of Na+,K+-ATPase.

J. W. Schneider, R. W. Mercer, M. Gilmore-Hebert, M. F. Utset, C. Lai, A. Greene, E. J. Benz

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

The isolation of multiple Na+,K+-ATPase cDNAs from rat brain has led to the discovery of a family of alpha-isoform genes. Using A1 (alpha), A2 (alpha+), and A3 (alpha III) Na+,K+-ATPase gene probes, we have analyzed the distribution of Na+,K+-ATPase mRNAs in adult and fetal rat tissues by RNA blot and hybridization histochemistry. A1 Na+,K+-ATPase mRNA was found ubiquitously among various tissues, with highest levels in transport epithelial and neural tissues. A2 mRNA was found in adult neural and muscle tissues, and A3 mRNA was found only in neural tissues and fetal heart muscle. Both A1 and A2 mRNAs were less abundant in fetal brain than in adult brain; in contrast, A3 mRNA was abundant at both stages. In situ mapping of brain areas that contain A3 mRNA suggests that this Na+,K+-ATPase isoenzyme is expressed predominantly by neural cells. Analysis of Na+,K+-ATPase proteins generated by cell-free translation of synthetic mRNAs suggests that the A3 protein has properties similar to A2 (alpha+).

Original languageEnglish (US)
Pages (from-to)284-288
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number1
StatePublished - Jan 1988

Fingerprint

Organ Specificity
Protein Biosynthesis
Protein Isoforms
Messenger RNA
varespladib methyl
Brain
Brain Mapping
Fetal Heart
sodium-translocating ATPase
Isoenzymes
Genes
Myocardium
Fetus
Epithelium
Complementary DNA
RNA
Muscles

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Tissue specificity, localization in brain, and cell-free translation of mRNA encoding the A3 isoform of Na+,K+-ATPase. / Schneider, J. W.; Mercer, R. W.; Gilmore-Hebert, M.; Utset, M. F.; Lai, C.; Greene, A.; Benz, E. J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, No. 1, 01.1988, p. 284-288.

Research output: Contribution to journalArticle

Schneider, J. W. ; Mercer, R. W. ; Gilmore-Hebert, M. ; Utset, M. F. ; Lai, C. ; Greene, A. ; Benz, E. J. / Tissue specificity, localization in brain, and cell-free translation of mRNA encoding the A3 isoform of Na+,K+-ATPase. In: Proceedings of the National Academy of Sciences of the United States of America. 1988 ; Vol. 85, No. 1. pp. 284-288.
@article{b5428eff9aa845bb9f41ae8bd94e8b2f,
title = "Tissue specificity, localization in brain, and cell-free translation of mRNA encoding the A3 isoform of Na+,K+-ATPase.",
abstract = "The isolation of multiple Na+,K+-ATPase cDNAs from rat brain has led to the discovery of a family of alpha-isoform genes. Using A1 (alpha), A2 (alpha+), and A3 (alpha III) Na+,K+-ATPase gene probes, we have analyzed the distribution of Na+,K+-ATPase mRNAs in adult and fetal rat tissues by RNA blot and hybridization histochemistry. A1 Na+,K+-ATPase mRNA was found ubiquitously among various tissues, with highest levels in transport epithelial and neural tissues. A2 mRNA was found in adult neural and muscle tissues, and A3 mRNA was found only in neural tissues and fetal heart muscle. Both A1 and A2 mRNAs were less abundant in fetal brain than in adult brain; in contrast, A3 mRNA was abundant at both stages. In situ mapping of brain areas that contain A3 mRNA suggests that this Na+,K+-ATPase isoenzyme is expressed predominantly by neural cells. Analysis of Na+,K+-ATPase proteins generated by cell-free translation of synthetic mRNAs suggests that the A3 protein has properties similar to A2 (alpha+).",
author = "Schneider, {J. W.} and Mercer, {R. W.} and M. Gilmore-Hebert and Utset, {M. F.} and C. Lai and A. Greene and Benz, {E. J.}",
year = "1988",
month = "1",
language = "English (US)",
volume = "85",
pages = "284--288",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "1",

}

TY - JOUR

T1 - Tissue specificity, localization in brain, and cell-free translation of mRNA encoding the A3 isoform of Na+,K+-ATPase.

AU - Schneider, J. W.

AU - Mercer, R. W.

AU - Gilmore-Hebert, M.

AU - Utset, M. F.

AU - Lai, C.

AU - Greene, A.

AU - Benz, E. J.

PY - 1988/1

Y1 - 1988/1

N2 - The isolation of multiple Na+,K+-ATPase cDNAs from rat brain has led to the discovery of a family of alpha-isoform genes. Using A1 (alpha), A2 (alpha+), and A3 (alpha III) Na+,K+-ATPase gene probes, we have analyzed the distribution of Na+,K+-ATPase mRNAs in adult and fetal rat tissues by RNA blot and hybridization histochemistry. A1 Na+,K+-ATPase mRNA was found ubiquitously among various tissues, with highest levels in transport epithelial and neural tissues. A2 mRNA was found in adult neural and muscle tissues, and A3 mRNA was found only in neural tissues and fetal heart muscle. Both A1 and A2 mRNAs were less abundant in fetal brain than in adult brain; in contrast, A3 mRNA was abundant at both stages. In situ mapping of brain areas that contain A3 mRNA suggests that this Na+,K+-ATPase isoenzyme is expressed predominantly by neural cells. Analysis of Na+,K+-ATPase proteins generated by cell-free translation of synthetic mRNAs suggests that the A3 protein has properties similar to A2 (alpha+).

AB - The isolation of multiple Na+,K+-ATPase cDNAs from rat brain has led to the discovery of a family of alpha-isoform genes. Using A1 (alpha), A2 (alpha+), and A3 (alpha III) Na+,K+-ATPase gene probes, we have analyzed the distribution of Na+,K+-ATPase mRNAs in adult and fetal rat tissues by RNA blot and hybridization histochemistry. A1 Na+,K+-ATPase mRNA was found ubiquitously among various tissues, with highest levels in transport epithelial and neural tissues. A2 mRNA was found in adult neural and muscle tissues, and A3 mRNA was found only in neural tissues and fetal heart muscle. Both A1 and A2 mRNAs were less abundant in fetal brain than in adult brain; in contrast, A3 mRNA was abundant at both stages. In situ mapping of brain areas that contain A3 mRNA suggests that this Na+,K+-ATPase isoenzyme is expressed predominantly by neural cells. Analysis of Na+,K+-ATPase proteins generated by cell-free translation of synthetic mRNAs suggests that the A3 protein has properties similar to A2 (alpha+).

UR - http://www.scopus.com/inward/record.url?scp=0023756643&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023756643&partnerID=8YFLogxK

M3 - Article

C2 - 2829175

AN - SCOPUS:0023756643

VL - 85

SP - 284

EP - 288

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 1

ER -