TNF α increases endothelial permeability via its effect on redistribution of VE cadherin induced by P38 MAPK activity

Jiangping Chang, Xudong Zhu, Zijuan Liu, Fiemu E. Nwariaku

Research output: Contribution to journalArticle

Abstract

Objective: To study the influence of tumor necrosis factor alpha (TNFa) on endothelial permeability and its mechanism. Methods: Human umbilical vein endothelial cell (HUVEC) monolayer permeability was measured by enzyme-linked immunosorbent assay for biotin-labeled albumin. Immunofluorescence, laser confocal microscopy, and Western immunoblotting were used to assess vascular endothelial (VE) cadherin distribution. Mitogen-activated protein kinase (MAPK) activity was determined by using functional kinase assay and was inhibited with the compounds SB202190 (P38 inhibitor) and PD98059 (ERK inhibitor). Results: TNFα significantly increased endothelial permeability and reduced the expression of membrane-associated VE cadherin. Furthermore, TNFα activated P38 MAPK and ERK MAPK compared with controls (P<0.05). Interestingly, SB202190 significantly reduced the effect of TNFα on endothelial permeability and cell-surface VE cadherin expression, but PD98059 did not. Conclusion: TNFα increases endothelial permeability by activating P38 MAPK and then inhibiting the expression of VE cadherin and its redistribution on HUVEC.

Original languageEnglish (US)
JournalJournal of Xi'an Jiaotong University (Medical Sciences)
Volume25
Issue number6
StatePublished - Dec 2004

Fingerprint

p38 Mitogen-Activated Protein Kinases
Permeability
Endothelial cells
Mitogen-Activated Protein Kinases
Human Umbilical Vein Endothelial Cells
Assays
Confocal Microscopy
Immunosorbents
Confocal microscopy
Biotin
Albumins
Monolayers
Phosphotransferases
Tumor Necrosis Factor-alpha
Fluorescent Antibody Technique
Membranes
Endothelial Cells
Western Blotting
Enzyme-Linked Immunosorbent Assay
cadherin 5

Keywords

  • Endothelium
  • Mitogen-activated protein kinase
  • Tumor necrosis factor alpha
  • Vascular endothelial cadherin

ASJC Scopus subject areas

  • Medicine(all)

Cite this

@article{cfb96abe62f54660913c383b1820438c,
title = "TNF α increases endothelial permeability via its effect on redistribution of VE cadherin induced by P38 MAPK activity",
abstract = "Objective: To study the influence of tumor necrosis factor alpha (TNFa) on endothelial permeability and its mechanism. Methods: Human umbilical vein endothelial cell (HUVEC) monolayer permeability was measured by enzyme-linked immunosorbent assay for biotin-labeled albumin. Immunofluorescence, laser confocal microscopy, and Western immunoblotting were used to assess vascular endothelial (VE) cadherin distribution. Mitogen-activated protein kinase (MAPK) activity was determined by using functional kinase assay and was inhibited with the compounds SB202190 (P38 inhibitor) and PD98059 (ERK inhibitor). Results: TNFα significantly increased endothelial permeability and reduced the expression of membrane-associated VE cadherin. Furthermore, TNFα activated P38 MAPK and ERK MAPK compared with controls (P<0.05). Interestingly, SB202190 significantly reduced the effect of TNFα on endothelial permeability and cell-surface VE cadherin expression, but PD98059 did not. Conclusion: TNFα increases endothelial permeability by activating P38 MAPK and then inhibiting the expression of VE cadherin and its redistribution on HUVEC.",
keywords = "Endothelium, Mitogen-activated protein kinase, Tumor necrosis factor alpha, Vascular endothelial cadherin",
author = "Jiangping Chang and Xudong Zhu and Zijuan Liu and Nwariaku, {Fiemu E.}",
year = "2004",
month = "12",
language = "English (US)",
volume = "25",
journal = "Journal of Xi'an Jiaotong University (Medical Sciences)",
issn = "1671-8259",
publisher = "Xi'an Jiaotong University",
number = "6",

}

TY - JOUR

T1 - TNF α increases endothelial permeability via its effect on redistribution of VE cadherin induced by P38 MAPK activity

AU - Chang, Jiangping

AU - Zhu, Xudong

AU - Liu, Zijuan

AU - Nwariaku, Fiemu E.

PY - 2004/12

Y1 - 2004/12

N2 - Objective: To study the influence of tumor necrosis factor alpha (TNFa) on endothelial permeability and its mechanism. Methods: Human umbilical vein endothelial cell (HUVEC) monolayer permeability was measured by enzyme-linked immunosorbent assay for biotin-labeled albumin. Immunofluorescence, laser confocal microscopy, and Western immunoblotting were used to assess vascular endothelial (VE) cadherin distribution. Mitogen-activated protein kinase (MAPK) activity was determined by using functional kinase assay and was inhibited with the compounds SB202190 (P38 inhibitor) and PD98059 (ERK inhibitor). Results: TNFα significantly increased endothelial permeability and reduced the expression of membrane-associated VE cadherin. Furthermore, TNFα activated P38 MAPK and ERK MAPK compared with controls (P<0.05). Interestingly, SB202190 significantly reduced the effect of TNFα on endothelial permeability and cell-surface VE cadherin expression, but PD98059 did not. Conclusion: TNFα increases endothelial permeability by activating P38 MAPK and then inhibiting the expression of VE cadherin and its redistribution on HUVEC.

AB - Objective: To study the influence of tumor necrosis factor alpha (TNFa) on endothelial permeability and its mechanism. Methods: Human umbilical vein endothelial cell (HUVEC) monolayer permeability was measured by enzyme-linked immunosorbent assay for biotin-labeled albumin. Immunofluorescence, laser confocal microscopy, and Western immunoblotting were used to assess vascular endothelial (VE) cadherin distribution. Mitogen-activated protein kinase (MAPK) activity was determined by using functional kinase assay and was inhibited with the compounds SB202190 (P38 inhibitor) and PD98059 (ERK inhibitor). Results: TNFα significantly increased endothelial permeability and reduced the expression of membrane-associated VE cadherin. Furthermore, TNFα activated P38 MAPK and ERK MAPK compared with controls (P<0.05). Interestingly, SB202190 significantly reduced the effect of TNFα on endothelial permeability and cell-surface VE cadherin expression, but PD98059 did not. Conclusion: TNFα increases endothelial permeability by activating P38 MAPK and then inhibiting the expression of VE cadherin and its redistribution on HUVEC.

KW - Endothelium

KW - Mitogen-activated protein kinase

KW - Tumor necrosis factor alpha

KW - Vascular endothelial cadherin

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M3 - Article

VL - 25

JO - Journal of Xi'an Jiaotong University (Medical Sciences)

JF - Journal of Xi'an Jiaotong University (Medical Sciences)

SN - 1671-8259

IS - 6

ER -