Toc receptor dimerization participates in the initiation of membrane translocation during protein import into chloroplasts

Jeonghwa Lee, Fei Wang, Danny J. Schnell

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The post-translational import of nucleus-encoded preproteins into chloroplasts occurs through multimeric translocons in the outer (Toc) and inner (Tic) membranes. The high fidelity of the protein import process is maintained by specific recognition of the transit peptide of preproteins by the coordinate activities of two homologous GTPase Toc receptors, Toc34 and Toc159. Structural and biochemical studies suggest that dimerization of the Toc receptors functions as a component of the mechanism to control access of preproteins to the membrane translocation channel of the translocon. We show that specific mutations that disrupted receptor dimerization in vitro reduced the rate of protein import in transgenic Arabidopsis compared with the wild type receptor. The mutations did not affect the GTPase activities of the receptors. Interestingly, these mutations did not decrease the initial preprotein binding at the receptors, but they reduced the efficiency of the transition from preprotein binding to membrane translocation. These data indicate that dimerization of receptors has a direct role in protein import and support a hypothesis in which receptor-receptor interactions participate in the initiation of membrane translocation of chloroplast preproteins as part of the molecular mechanism of GTP-regulated protein import.

Original languageEnglish (US)
Pages (from-to)31130-31141
Number of pages12
JournalJournal of Biological Chemistry
Volume284
Issue number45
DOIs
StatePublished - Nov 6 2009

Fingerprint

Dimerization
Protein Transport
Chloroplasts
Membranes
GTP Phosphohydrolases
Mutation
Proteins
Tics
Guanosine Triphosphate
Ion Channels
Arabidopsis
Access control
Peptides

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Toc receptor dimerization participates in the initiation of membrane translocation during protein import into chloroplasts. / Lee, Jeonghwa; Wang, Fei; Schnell, Danny J.

In: Journal of Biological Chemistry, Vol. 284, No. 45, 06.11.2009, p. 31130-31141.

Research output: Contribution to journalArticle

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