@inbook{2a95f75b47904acab283cd9d51ff88d8,
title = "Top-down mass spectrometry: Proteomics to proteoforms",
abstract = "This chapter highlights many of the fundamental concepts and technologies in the field of top-down mass spectrometry (TDMS), and provides numerous examples of contributions that TD is making in biology, biophysics, and clinical investigations. TD workflows include variegated steps that may include non-specific or targeted preparative strategies, orthogonal liquid chromatography techniques, analyte ionization, mass analysis, tandem mass spectrometry (MS/MS) and informatics procedures. This diversity of experimental designs has evolved to manage the large dynamic range of protein expression and diverse physiochemical properties of proteins in proteome investigations, tackle proteoform microheterogeneity, as well as determine structure and composition of gas-phase proteins and protein assemblies.",
keywords = "Alternative splicing, Bottom-up, Charge reduction, Chromatography, Data-dependent, Data-independent, Electrospray ionization, FT-ICR, Fourier transform, Hydrogen/ deuterium exchange, Informatics, Label-free quantitation, Mass spectrometry, Membrane proteins, Native MS, Orbitrap, Post-translational modifications, Protein, Proteoform, Proteomics, Quadrupole ion trap, Reversed-phase, Review, SILAC, SNPs, Supercharging, Tandem MS, Time-of-flight, Topdown",
author = "Patrie, {Steven M.}",
note = "Funding Information: This work was supported by the National Institute of General Medical Sciences of the National Institutes of Health under award number 1R01GM115739-01A1. Any opinions, findings, conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Institutes of Health. This work was also supported by the Multiple Sclerosis Society [PP-1503-04034], The Darrel K. Royal Research Fund for Alzheimer{\textquoteright}s Disease [48680-DKR], The Texas Alzheimer{\textquoteright}s Research and Care Consortium Investigator Grant Program [354091], and the UT System Neuroscience and Neurotechnology Research Institute [363027]. Publisher Copyright: {\textcopyright} Springer International Publishing Switzerland 2016.",
year = "2016",
doi = "10.1007/978-3-319-41448-5_8",
language = "English (US)",
series = "Advances in Experimental Medicine and Biology",
publisher = "Springer New York LLC",
pages = "171--200",
booktitle = "Advances in Experimental Medicine and Biology",
}