Abstract
This chapter highlights many of the fundamental concepts and technologies in the field of top-down mass spectrometry (TDMS), and provides numerous examples of contributions that TD is making in biology, biophysics, and clinical investigations. TD workflows include variegated steps that may include non-specific or targeted preparative strategies, orthogonal liquid chromatography techniques, analyte ionization, mass analysis, tandem mass spectrometry (MS/MS) and informatics procedures. This diversity of experimental designs has evolved to manage the large dynamic range of protein expression and diverse physiochemical properties of proteins in proteome investigations, tackle proteoform microheterogeneity, as well as determine structure and composition of gas-phase proteins and protein assemblies.
| Original language | English (US) |
|---|---|
| Title of host publication | Advances in Experimental Medicine and Biology |
| Publisher | Springer New York LLC |
| Pages | 171-200 |
| Number of pages | 30 |
| Volume | 919 |
| DOIs | |
| State | Published - 2016 |
Publication series
| Name | Advances in Experimental Medicine and Biology |
|---|---|
| Volume | 919 |
| ISSN (Print) | 00652598 |
| ISSN (Electronic) | 22148019 |
Fingerprint
Keywords
- Alternative splicing
- Bottom-up
- Charge reduction
- Chromatography
- Data-dependent
- Data-independent
- Electrospray ionization
- Fourier transform
- FT-ICR
- Hydrogen/ deuterium exchange
- Informatics
- Label-free quantitation
- Mass spectrometry
- Membrane proteins
- Native MS
- Orbitrap
- Post-translational modifications
- Protein
- Proteoform
- Proteomics
- Quadrupole ion trap
- Reversed-phase
- Review
- SILAC
- SNPs
- Supercharging
- Tandem MS
- Time-of-flight
- Topdown
ASJC Scopus subject areas
- Medicine(all)
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Top-down mass spectrometry : Proteomics to proteoforms. / Patrie, Steven M.
Advances in Experimental Medicine and Biology. Vol. 919 Springer New York LLC, 2016. p. 171-200 (Advances in Experimental Medicine and Biology; Vol. 919).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Top-down mass spectrometry
T2 - Proteomics to proteoforms
AU - Patrie, Steven M.
PY - 2016
Y1 - 2016
N2 - This chapter highlights many of the fundamental concepts and technologies in the field of top-down mass spectrometry (TDMS), and provides numerous examples of contributions that TD is making in biology, biophysics, and clinical investigations. TD workflows include variegated steps that may include non-specific or targeted preparative strategies, orthogonal liquid chromatography techniques, analyte ionization, mass analysis, tandem mass spectrometry (MS/MS) and informatics procedures. This diversity of experimental designs has evolved to manage the large dynamic range of protein expression and diverse physiochemical properties of proteins in proteome investigations, tackle proteoform microheterogeneity, as well as determine structure and composition of gas-phase proteins and protein assemblies.
AB - This chapter highlights many of the fundamental concepts and technologies in the field of top-down mass spectrometry (TDMS), and provides numerous examples of contributions that TD is making in biology, biophysics, and clinical investigations. TD workflows include variegated steps that may include non-specific or targeted preparative strategies, orthogonal liquid chromatography techniques, analyte ionization, mass analysis, tandem mass spectrometry (MS/MS) and informatics procedures. This diversity of experimental designs has evolved to manage the large dynamic range of protein expression and diverse physiochemical properties of proteins in proteome investigations, tackle proteoform microheterogeneity, as well as determine structure and composition of gas-phase proteins and protein assemblies.
KW - Alternative splicing
KW - Bottom-up
KW - Charge reduction
KW - Chromatography
KW - Data-dependent
KW - Data-independent
KW - Electrospray ionization
KW - Fourier transform
KW - FT-ICR
KW - Hydrogen/ deuterium exchange
KW - Informatics
KW - Label-free quantitation
KW - Mass spectrometry
KW - Membrane proteins
KW - Native MS
KW - Orbitrap
KW - Post-translational modifications
KW - Protein
KW - Proteoform
KW - Proteomics
KW - Quadrupole ion trap
KW - Reversed-phase
KW - Review
KW - SILAC
KW - SNPs
KW - Supercharging
KW - Tandem MS
KW - Time-of-flight
KW - Topdown
UR - http://www.scopus.com/inward/record.url?scp=85006379813&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85006379813&partnerID=8YFLogxK
U2 - 10.1007/978-3-319-41448-5_8
DO - 10.1007/978-3-319-41448-5_8
M3 - Chapter
C2 - 27975217
AN - SCOPUS:85006379813
VL - 919
T3 - Advances in Experimental Medicine and Biology
SP - 171
EP - 200
BT - Advances in Experimental Medicine and Biology
PB - Springer New York LLC
ER -