Toward a Comprehensive Temperature-Sensitive Mutant Repository of the Essential Genes of Saccharomyces cerevisiae

Shay Ben-Aroya, Candice Coombes, Teresa Kwok, Kathryn A O'Donnell-Mendell, Jef D. Boeke, Philip Hieter

Research output: Contribution to journalArticle

138 Citations (Scopus)

Abstract

The Saccharomyces cereivisiae gene deletion project revealed that approximately 20% of yeast genes are required for viability. The analysis of essential genes traditionally relies on conditional mutants, typically temperature-sensitive (ts) alleles. We developed a systematic approach (termed "diploid shuffle") useful for generating a ts allele for each essential gene in S. cerevisiae and for improved genetic manipulation of mutant alleles and gene constructs in general. Importantly, each ts allele resides at its normal genomic locus, flanked by specific cognate UPTAG and DNTAG bar codes. A subset of 250 ts mutants, including ts alleles for all uncharacterized essential genes and prioritized for genes with human counterparts, is now ready for distribution. The importance of this collection is demonstrated by biochemical and genetic screens that reveal essential genes involved in RNA processing and maintenance of chromosomal stability.

Original languageEnglish (US)
Pages (from-to)248-258
Number of pages11
JournalMolecular Cell
Volume30
Issue number2
DOIs
StatePublished - Apr 25 2008

Fingerprint

Essential Genes
Saccharomyces cerevisiae
Alleles
Temperature
Genes
Chromosomal Instability
Saccharomyces
Gene Deletion
Diploidy
Automatic Data Processing
Molecular Biology
Yeasts
Maintenance
RNA

Keywords

  • DNA
  • RNA

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Toward a Comprehensive Temperature-Sensitive Mutant Repository of the Essential Genes of Saccharomyces cerevisiae. / Ben-Aroya, Shay; Coombes, Candice; Kwok, Teresa; O'Donnell-Mendell, Kathryn A; Boeke, Jef D.; Hieter, Philip.

In: Molecular Cell, Vol. 30, No. 2, 25.04.2008, p. 248-258.

Research output: Contribution to journalArticle

Ben-Aroya, Shay ; Coombes, Candice ; Kwok, Teresa ; O'Donnell-Mendell, Kathryn A ; Boeke, Jef D. ; Hieter, Philip. / Toward a Comprehensive Temperature-Sensitive Mutant Repository of the Essential Genes of Saccharomyces cerevisiae. In: Molecular Cell. 2008 ; Vol. 30, No. 2. pp. 248-258.
@article{69aa8f416352456285de1081b2fb9070,
title = "Toward a Comprehensive Temperature-Sensitive Mutant Repository of the Essential Genes of Saccharomyces cerevisiae",
abstract = "The Saccharomyces cereivisiae gene deletion project revealed that approximately 20{\%} of yeast genes are required for viability. The analysis of essential genes traditionally relies on conditional mutants, typically temperature-sensitive (ts) alleles. We developed a systematic approach (termed {"}diploid shuffle{"}) useful for generating a ts allele for each essential gene in S. cerevisiae and for improved genetic manipulation of mutant alleles and gene constructs in general. Importantly, each ts allele resides at its normal genomic locus, flanked by specific cognate UPTAG and DNTAG bar codes. A subset of 250 ts mutants, including ts alleles for all uncharacterized essential genes and prioritized for genes with human counterparts, is now ready for distribution. The importance of this collection is demonstrated by biochemical and genetic screens that reveal essential genes involved in RNA processing and maintenance of chromosomal stability.",
keywords = "DNA, RNA",
author = "Shay Ben-Aroya and Candice Coombes and Teresa Kwok and O'Donnell-Mendell, {Kathryn A} and Boeke, {Jef D.} and Philip Hieter",
year = "2008",
month = "4",
day = "25",
doi = "10.1016/j.molcel.2008.02.021",
language = "English (US)",
volume = "30",
pages = "248--258",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "2",

}

TY - JOUR

T1 - Toward a Comprehensive Temperature-Sensitive Mutant Repository of the Essential Genes of Saccharomyces cerevisiae

AU - Ben-Aroya, Shay

AU - Coombes, Candice

AU - Kwok, Teresa

AU - O'Donnell-Mendell, Kathryn A

AU - Boeke, Jef D.

AU - Hieter, Philip

PY - 2008/4/25

Y1 - 2008/4/25

N2 - The Saccharomyces cereivisiae gene deletion project revealed that approximately 20% of yeast genes are required for viability. The analysis of essential genes traditionally relies on conditional mutants, typically temperature-sensitive (ts) alleles. We developed a systematic approach (termed "diploid shuffle") useful for generating a ts allele for each essential gene in S. cerevisiae and for improved genetic manipulation of mutant alleles and gene constructs in general. Importantly, each ts allele resides at its normal genomic locus, flanked by specific cognate UPTAG and DNTAG bar codes. A subset of 250 ts mutants, including ts alleles for all uncharacterized essential genes and prioritized for genes with human counterparts, is now ready for distribution. The importance of this collection is demonstrated by biochemical and genetic screens that reveal essential genes involved in RNA processing and maintenance of chromosomal stability.

AB - The Saccharomyces cereivisiae gene deletion project revealed that approximately 20% of yeast genes are required for viability. The analysis of essential genes traditionally relies on conditional mutants, typically temperature-sensitive (ts) alleles. We developed a systematic approach (termed "diploid shuffle") useful for generating a ts allele for each essential gene in S. cerevisiae and for improved genetic manipulation of mutant alleles and gene constructs in general. Importantly, each ts allele resides at its normal genomic locus, flanked by specific cognate UPTAG and DNTAG bar codes. A subset of 250 ts mutants, including ts alleles for all uncharacterized essential genes and prioritized for genes with human counterparts, is now ready for distribution. The importance of this collection is demonstrated by biochemical and genetic screens that reveal essential genes involved in RNA processing and maintenance of chromosomal stability.

KW - DNA

KW - RNA

UR - http://www.scopus.com/inward/record.url?scp=43049146539&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=43049146539&partnerID=8YFLogxK

U2 - 10.1016/j.molcel.2008.02.021

DO - 10.1016/j.molcel.2008.02.021

M3 - Article

C2 - 18439903

AN - SCOPUS:43049146539

VL - 30

SP - 248

EP - 258

JO - Molecular Cell

JF - Molecular Cell

SN - 1097-2765

IS - 2

ER -