Toward an understanding of the complete NCX1 lifetime in the cardiac sarcolemma

Donald W. Hilgemann, Mei Jung Lin, Michael Fine, Gary Frazier, Hao Ran Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

The density of Na/Ca exchangers (NCX1) in the cardiac sarcolemma, like all plasma membrane proteins, will be influenced by (and ultimately determined by) the function of membrane insertion and retrieval processes (i.e., exo- and endocytic mechanisms). Progress in understanding these processes in cardiac muscle faces many biological and methodological complexities and hurdles. As described here, we are attempting to overcome these hurdles to study more adequately the assembly and disassembly of the cardiac sarcolemma, in general, and the control of NCX1 by membrane trafficking processes in particular. First, we have developed improved noninvasive methods to monitor the cellular capacitance of cardiac tissue (NIC) over periods of hours. Thus, we can study long-term changes of total membrane area. Second, we have developed mice that express fusion proteins of NCX1 with the pHluorin green protein. Thus, we can determine the membrane disposition of NCX1, and changes thereof, on-line in intact cardiac muscle.

Original languageEnglish (US)
Title of host publicationSodium Calcium Exchange
Subtitle of host publicationA Growing Spectrum of Pathophysiological Implications: Proceedings of the 6th International Conference on Sodium Calcium Exchange
PublisherSpringer Science and Business Media, LLC
Pages345-352
Number of pages8
ISBN (Print)9781461447559
DOIs
StatePublished - 2013

Publication series

NameAdvances in Experimental Medicine and Biology
Volume961
ISSN (Print)0065-2598

Keywords

  • Cardiac sarcolemma
  • Electrophysiology
  • Endocytosis
  • Exocytosis
  • NCX1

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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