TY - GEN
T1 - Toward an understanding of the complete NCX1 lifetime in the cardiac sarcolemma
AU - Hilgemann, Donald W.
AU - Lin, Mei Jung
AU - Fine, Michael
AU - Frazier, Gary
AU - Wang, Hao Ran
N1 - Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2013
Y1 - 2013
N2 - The density of Na/Ca exchangers (NCX1) in the cardiac sarcolemma, like all plasma membrane proteins, will be influenced by (and ultimately determined by) the function of membrane insertion and retrieval processes (i.e., exo- and endocytic mechanisms). Progress in understanding these processes in cardiac muscle faces many biological and methodological complexities and hurdles. As described here, we are attempting to overcome these hurdles to study more adequately the assembly and disassembly of the cardiac sarcolemma, in general, and the control of NCX1 by membrane trafficking processes in particular. First, we have developed improved noninvasive methods to monitor the cellular capacitance of cardiac tissue (NIC) over periods of hours. Thus, we can study long-term changes of total membrane area. Second, we have developed mice that express fusion proteins of NCX1 with the pHluorin green protein. Thus, we can determine the membrane disposition of NCX1, and changes thereof, on-line in intact cardiac muscle.
AB - The density of Na/Ca exchangers (NCX1) in the cardiac sarcolemma, like all plasma membrane proteins, will be influenced by (and ultimately determined by) the function of membrane insertion and retrieval processes (i.e., exo- and endocytic mechanisms). Progress in understanding these processes in cardiac muscle faces many biological and methodological complexities and hurdles. As described here, we are attempting to overcome these hurdles to study more adequately the assembly and disassembly of the cardiac sarcolemma, in general, and the control of NCX1 by membrane trafficking processes in particular. First, we have developed improved noninvasive methods to monitor the cellular capacitance of cardiac tissue (NIC) over periods of hours. Thus, we can study long-term changes of total membrane area. Second, we have developed mice that express fusion proteins of NCX1 with the pHluorin green protein. Thus, we can determine the membrane disposition of NCX1, and changes thereof, on-line in intact cardiac muscle.
KW - Cardiac sarcolemma
KW - Electrophysiology
KW - Endocytosis
KW - Exocytosis
KW - NCX1
UR - http://www.scopus.com/inward/record.url?scp=84873596374&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84873596374&partnerID=8YFLogxK
U2 - 10.1007/978-1-4614-4756-6_29
DO - 10.1007/978-1-4614-4756-6_29
M3 - Conference contribution
C2 - 23224893
AN - SCOPUS:84873596374
SN - 9781461447559
T3 - Advances in Experimental Medicine and Biology
SP - 345
EP - 352
BT - Sodium Calcium Exchange
PB - Springer Science and Business Media, LLC
ER -