Toxicity of indium-111 on the radiolabeled lymphocyte

E. P. Balaban, T. R. Simon, E. P. Frenkel

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The radiolabeling of lymphocytes with 111In has resulted in detectable toxic changes in the cells. The mechanisms of toxicity for lymphocytes have been related to the label's radioactivity and to the chelator used to mediate the intracellular localization. These mechanisms were examined by assessing cellular function with mitogen-mediated blastogenesis after labeling lymphocytes with either the chelator (tropolone) alone, 111In complexed with tropolone, or cadmium (the decay product of 111In) complexed with tropolone. Successful lymphocyte labeling with 111In was shown to be dependent upon the concentration of the chelator (tropolone). Increasing concentrations of tropolone inhibited lymphocyte function to a variable degree. Further reduction in cellular function was detected after incorporation of a constant amount of 111In or 111In's decay product, cadmium. Lymphocyte function was decreased by these two labels in a parallel linear manner. This same toxic effect was seen after labeling with small constant amounts of tropolone and increasing quantities of 111In or cadmium. Thus, although both the required chelator and the radiobiologic exposure have a deleterious effect on the lymphocyte, significant lymphocyte toxicity appears to result from the metal-to-cell interaction as a result of the metal decay product (cadmium).

Original languageEnglish (US)
Pages (from-to)229-233
Number of pages5
JournalJournal of Nuclear Medicine
Volume28
Issue number2
StatePublished - 1987

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Indium
Tropolone
Lymphocytes
Chelating Agents
Cadmium
Poisons
Metals
Lymphocyte Activation
Mitogens
Cell Communication
Radioactivity

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology

Cite this

Balaban, E. P., Simon, T. R., & Frenkel, E. P. (1987). Toxicity of indium-111 on the radiolabeled lymphocyte. Journal of Nuclear Medicine, 28(2), 229-233.

Toxicity of indium-111 on the radiolabeled lymphocyte. / Balaban, E. P.; Simon, T. R.; Frenkel, E. P.

In: Journal of Nuclear Medicine, Vol. 28, No. 2, 1987, p. 229-233.

Research output: Contribution to journalArticle

Balaban, EP, Simon, TR & Frenkel, EP 1987, 'Toxicity of indium-111 on the radiolabeled lymphocyte', Journal of Nuclear Medicine, vol. 28, no. 2, pp. 229-233.
Balaban EP, Simon TR, Frenkel EP. Toxicity of indium-111 on the radiolabeled lymphocyte. Journal of Nuclear Medicine. 1987;28(2):229-233.
Balaban, E. P. ; Simon, T. R. ; Frenkel, E. P. / Toxicity of indium-111 on the radiolabeled lymphocyte. In: Journal of Nuclear Medicine. 1987 ; Vol. 28, No. 2. pp. 229-233.
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